Flexible Raman biosensing platform for low-cost health diagnostics

用于低成本健康诊断的灵活拉曼生物传感平台

• 批准号: EP/R011230/1
• 负责人: James Wilkinson
• 金额: $ 123.96万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR011230%2F1
• 关键词: Flexible; Raman; biosensing; platform; low

Highly specific, sensitive sensors interfaced with portable, easy-to use, low-cost instruments are needed for rapid point-of-care infection diagnostics, and will lead to better targeted therapy, shorter time to treatment and reduced morbidity. In this project, we propose to realise a generic, flexible, compact sensing platform with high sensitivity and selectivity. The system will comprise a low-cost instrument employing cheap, disposable generic sensor chips that can be readily committed to specific analytes, from small molecules to proteins and DNA, by using conventional surface modification techniques. Paper-based fluidics will be used to deliver analytes straightforwardly from a drop to the sensor surface or, where required, fluidic microsystems may be easily integrated. The sensing system will be demonstrated with clinical samples from patients who have been exposed to controlled infection to whooping cough and for the analysis of priority pathogens such as ebola and plague. This proposal builds upon our recent work on waveguide-enhanced Raman spectroscopy (WERS) to realise a sensor chip which shows surface enhancements comparable to those of surface enhanced Raman spectroscopy (SERS) with improved application flexibility and manufacturability, and upon joint work between Dstl and Chemistry on surface enhanced Raman approaches to bioanalyte detection. The proposed biosensing platform will have widespread application at the point of care for patients, in the detection of infection and the diagnosis of disease, and for broader applications such as environmental monitoring and security, and will be flexibly configurable for specific settings and analytical challenges. The desktop instrument, employing plug-in disposable sensor chips with simple operation will be appropriate for use in the GP's surgery, the ward, or in remote communities.

需要高度特异性、灵敏的传感器与便携式、易于使用、低成本的仪器相结合，以进行快速的护理点感染诊断，并将导致更好的靶向治疗、更短的治疗时间和更低的发病率。在这个项目中，我们建议实现一个通用的，灵活的，紧凑的传感平台，具有高灵敏度和选择性。该系统将包括一个低成本的仪器，采用廉价的，一次性的通用传感器芯片，可以很容易地致力于特定的分析物，从小分子到蛋白质和DNA，通过使用传统的表面改性技术。纸基射流将用于直接从液滴向传感器表面输送分析物，或者在需要时，可以容易地集成流体微系统。该传感系统将使用来自暴露于百日咳控制感染的患者的临床样本进行演示，并用于分析埃博拉和鼠疫等优先病原体。这一建议建立在我们最近的工作波导增强拉曼光谱（WERS），以实现传感器芯片，显示表面增强与表面增强拉曼光谱（Sers）具有改进的应用灵活性和可制造性，并在DSTL和化学之间的联合工作表面增强拉曼方法，以生物分析物检测。拟议的生物传感平台将在患者护理点、感染检测和疾病诊断以及环境监测和安全等更广泛的应用中具有广泛的应用，并将针对特定设置和分析挑战进行灵活配置。台式仪器，采用插入式一次性传感器芯片，操作简单，将适用于家庭医生的手术，病房，或在偏远社区使用。

项目成果

Study of waveguide background at visible wavelengths for on-chip nanoscopy.

• DOI: 10.1364/oe.420844
• 发表时间: 2021-01
• 期刊: Optics express
• 影响因子: 3.8
• 作者: D. Coucheron;Ø. Helle;J. Wilkinson;G. S. Murugan;Carlos Dom'inguez;H. Angelskår;B. Ahluwalia

Monitoring the efficient bioconjugation of small molecules

监测小分子的有效生物共轭

• 发表时间: 2020
• 作者: Marti A

Multiframe-based non-local means denoising for Raman spectra

• DOI: 10.1016/j.saa.2024.123931
• 发表时间: 2024-01-31
• 期刊: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
• 影响因子: 4.4
• 作者: Liu，Zhen;Ettabib，Mohamed A.;Zervas，Michalis N.
• 通讯作者: Zervas，Michalis N.

Effect of Scattering Loss on Optimization of Waveguide Enhanced Raman Spectroscopy

• DOI: 10.1109/jlt.2023.3337390
• 发表时间: 2024-04-01
• 期刊: JOURNAL OF LIGHTWAVE TECHNOLOGY
• 影响因子: 4.7
• 作者: Liu，Zhen;Ettabib，Mohamed. A.;Zervas，Michalis. N.
• 通讯作者: Zervas，Michalis. N.

Accurate dipole radiation model for waveguide grating couplers

波导光栅耦合器的精确偶极子辐射模型

• DOI: 10.1016/j.rinp.2023.106526
• 发表时间: 2023
• 期刊: Results in Physics
• 影响因子: 5.3
• 作者: Liu Z