Combining the Strengths of Mid-IR and Raman Spectroscopies on Single Chip for Rapid Bedside Biomarker Diagnostics

在单芯片上结合中红外和拉曼光谱的优势，实现快速床旁生物标志物诊断

• 批准号: EP/S03109X/1
• 负责人: Senthil Murugan Ganapathy
• 金额: $ 102.6万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FS03109X%2F1
• 关键词: Combining; Strengths; Mid; IR; Raman

There is a pressing need for diagnostic tools that can produce results quickly from patients' bedsides and in doctors' surgeries. Rapid, accurate results will allow rapid therapeutic decisions and save lives at reduced cost. In contrast, existing technologies require transfer of samples to centrally located laboratories equipped with sophisticated instruments, and highly skilled personnel.Bedside diagnostics using simplified, compact, versatile and efficient tools providing analysis results within a few minutes will therefore be a boon for many critically ill patients. In this project, we propose to develop two-in-one attenuated total reflection (ATR)/Raman chips that are compact, mass-producible, affordable, reliable, user-friendly and highly sensitive. The availability of such chips will enable the full potential and complementary nature of mid-IR molecular fingerprint and Raman spectroscopies to be exploited for bedside point-of-care diagnosis of critically ill patients who require rapid therapeutic decisions, meeting the ASSURED criteria set by the World Health Organisation (WHO).For example, provision of rapid diagnostic information will be invaluable for preterm infants (24-30 weeks gestation), for whom treatment decisions must be made as soon after birth as possible. Due to their lung immaturity, such infants are at high risk of suffering from neonatal Respiratory Distress Syndrome (nRDS), which has a high rates of mortality and morbidity with a major long-term economic burden on healthcare services. Using nRDS as an exemplar, we propose to develop a compact, versatile, rapid and easily operable bedside diagnostic tool for the next-generation bedside point-of-care to provide a predictive diagnostic test for nRDS to inform treatment options. The diagnostic device platform proposed here combines the complementary capabilities of fingerprint Mid-IR and Raman spectroscopies, each of which has been shown independently to be powerful biodiagnostic tool for specific biomarkers. In addition, we will employ a unique signal enhancement strategy that will simultaneously benefit both IR and Raman spectroscopies and significantly enhance their sensitivities. This new photonic technology and the portable diagnostic device proposed will not only underpin next-generation biomedical diagnostic applications but will also have major impacts in environmental monitoring and sensing including water pollution monitoring and trace toxic gas sensing.

迫切需要一种诊断工具，可以在病人的床边和医生的手术中迅速产生结果。快速、准确的结果将有助于快速做出治疗决定，并以更低的成本挽救生命。相比之下，现有技术需要将样品转移到配备精密仪器和高技能人员的中心实验室。因此，使用简化、紧凑、通用和高效的工具在几分钟内提供分析结果的床边诊断将是许多危重患者的福音。在这个项目中，我们建议开发二合一衰减全反射(ATR)/拉曼芯片，该芯片紧凑，可批量生产，价格合理，可靠，用户友好且高灵敏度。这种芯片的可用性将使中红外分子指纹和拉曼光谱的全部潜力和互补性质得以充分利用，用于需要快速治疗决策的危重患者的床边即时诊断，符合世界卫生组织（who）确定的标准。例如，提供快速诊断信息对早产儿（妊娠24-30周）将是非常宝贵的，对他们必须在出生后尽快作出治疗决定。由于肺发育不成熟，这些婴儿患新生儿呼吸窘迫综合征（nRDS）的风险很高，这种疾病的死亡率和发病率很高，对医疗保健服务造成了重大的长期经济负担。以nRDS为例，我们建议为下一代床边护理点开发一种紧凑、通用、快速和易于操作的床边诊断工具，为nRDS提供预测性诊断测试，为治疗方案提供信息。本文提出的诊断设备平台结合了指纹中红外光谱和拉曼光谱的互补功能，每一个都被证明是特定生物标志物的强大生物诊断工具。此外，我们将采用一种独特的信号增强策略，同时使红外和拉曼光谱受益，并显着提高其灵敏度。这种新型光子技术和便携式诊断设备不仅将为下一代生物医学诊断应用奠定基础，而且将在环境监测和传感领域产生重大影响，包括水污染监测和痕量有毒气体传感。

项目成果

Prediction of Neonatal Respiratory Distress Biomarker Concentration by Application of Machine Learning to Mid-Infrared Spectra.

• DOI: 10.3390/s22051744
• 发表时间: 2022-02-23
• 期刊: Sensors (Basel, Switzerland)
• 作者: Ahmed W;Veluthandath AV;Rowe DJ;Madsen J;Clark HW;Postle AD;Wilkinson JS;Murugan GS
• 通讯作者: Murugan GS

ATR based Infrared Spectroscopy for the diagnosis of Neonatal Respiratory Distress Syndrome

基于 ATR 的红外光谱诊断新生儿呼吸窘迫综合征

• 发表时间: 2021
• 作者: Ahmed W

Design of photonic nanojets on a silicon chip

硅芯片上光子纳米喷射的设计

• DOI: 10.1117/12.2603613
• 发表时间: 2021
• 作者: Veluthandath A

Amorphous germanium waveguides for medical diagnostics using mid-infrared spectroscopy

使用中红外光谱进行医疗诊断的非晶锗波导

• DOI: 10.1117/12.2648868
• 发表时间: 2023
• 作者: Osborne E

Mid-IR Spectroscopy: On-chip Devices and Biodiagnostic Applications (Invited)

中红外光谱：片上器件和生物诊断应用（特邀）

• 发表时间: 2020
• 作者: Senthil Murugan G