HTLV RETROVIRUS INFECTION IN HAITIANS WITH AIDS

年爆发了获得性免疫缺陷综合症(艾滋病)。 佛罗里达州迈阿密的海地居民。这些艾滋病患者有 细胞介导的免疫缺陷与一种 增加对肺孢子虫等机会性感染的易感性 卡氏肺吸虫、弓形虫和播散性疱疹病毒。实验室 数据显示，海地艾滋病患者有T淋巴细胞亚群 异常、丙种球蛋白异常和循环免疫复合体 与患有艾滋病的男性同性恋者中发现的类似。正如在中提到的 患有艾滋病的男性同性恋者，海地艾滋病患者明显增加 卡波西肉瘤发生率(KS)。我们还描述了一种婴儿形式的 母亲有明显免疫力的13名海地儿童中的艾滋病 异常现象。最近，其中两名婴儿被发现患有KS 并代表了这个国家关于儿童KS的第一次报告。海地人 也是人类T细胞白血病/淋巴瘤的相对高发人群 病毒(HTLV)感染(28%)，通过血清抗体检测 HTLV特异性细胞表面抗原。HTLV在海地的存在 男性同性恋者人群中HTLV感染与艾滋病的关系 提示HTLV在艾滋病的发病机制中可能起一定作用 海地人。为了研究这一人群中的HTLV感染，这是第一次 有必要确定检测方法并研究其发病机制 在艾滋病患者和接触者中感染HTLV的可能性。目前的建议 用于研究HTLV-病毒-宿主的相互作用。我们将对HTLV进行量化 日本血吸虫外周血白细胞多肽和核酸序列 抗体阳性和阴性的海地人。患者的组织和 没有艾滋病的人也将在活组织检查和尸检中检查HTLV 材料。HTLV的分离及其免疫学研究 现有HTLV抗体阳性海地血清将提供 关于患者的血清学反应性与 HTLV基因分化。对HTLV的免疫反应将在 免疫球蛋白分类、时间表达和病毒粒子的术语 参与刺激自然免疫的多肽和/或前体。 这些免疫反应将与临床情况相关，以 确定潜在的预后因素。一旦足够敏感 并对特定的分析进行了表征，从海地人那里收集的血清 迈阿密作为未来多中心疾控中心协作病例控制的一部分 研究和来自医院和实验室的工作人员将被检查以寻找证据 HTLV感染的可能性。

An outbreak of acquired immunodeficiency syndrome (AIDS) is occurring in Haitian residents in Miami, Florida. These AIDS patients have the characteristic defects in cell mediated immunity associated with an increased susceptibility to opportunistic infections such as Pneumocystis carinii (PC), Toxoplasm gondii and disseminated Herpesvirus. Laboratory data reveals that Haitian patients with AIDS have T-lymphocyte subset abnormalities, dysgammaglobulinemias, and circulating immune complexes similar to those noted in male homosexuals with AIDS. As has been noted in male homosexuals with AIDS, Haitians with AIDS have a markedly increased rate of Kaposi's sarcoma (KS). We have also described an infant form of AIDS in 13 Haitian children born to mothers with marked immunologic abnormalities. Recently two of these infants have been found to have KS and represent the first reports of childhood KS in this country. Haitians also have a relatively high incidence of human T-cell leukemia/lymphoma virus (HTLV) infections (28%) as measured by serum antibody to HTLV-specific cell surface antigens. The presence of HTLV in the Haitian population and the association of HTLV with AIDS in male homosexuals suggest that HTLV may play some role in the pathogenesis of AIDS among Haitians. In order to study HTLV infection in this population it is first necessary to define detection methods and to investigate the pathogenesis of HTLV infection in both AIDS patients and contacts. The present proposal is for the study of HTLV-virus-host interactions. We will quantitate HTLV polypeptides and nucleic acid sequences in peripheral blood leukocytes from antibody-positive and negative Haitians. Tissues from patients with and without AIDS will also be examined for HTLV both in biopsy and autopsy materials. Isolation of HTLV as well as immunologic studies of already existing HTLV antibody-positive Haitian sera will be undertaken to provide information on the correlation between patient's serologic reactivity and HTLV genetic divergence. Immune responses to HTLV will be measured in terms of immunoglobulin classes, temporal expression and the virion polypeptides and/or precursors involved in stimulating natural immunity. These immune responses will be correlated with clinical conditions to determine potential prognostic factors. as soon as adequately sensitive and specific assays are characterized, sera collected from Haitians in Miami as part of a prospective multicenter CDC collaborative case control study and from hospital and laboratory staff will be examined for evidence of HTLV infection.