CBET-EPSRC: Developing Standardized Cell-Free Platforms for Rapid Prototyping of Complex Synthetic Biology Circuits and Pathways

CBET-EPSRC：开发标准化无细胞平台，用于复杂合成生物学电路和通路的快速原型制作

• 批准号: EP/T013788/1
• 负责人: Paul Freemont
• 金额: $ 64.68万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FT013788%2F1
• 关键词: CBET; EPSRC; Developing; Standardized; Cell

Synthetic biology is an emerging field of engineering that aims to establish a systematic framework for the design of biological systems based on a 'bottom-up' approach for the reconstruction of complex bio-molecular systems. The application of an engineering approach to design is attractive, as many engineering parallels can be identified in living systems. However, biological systems are highly complex and dynamic and difficult to engineer. Rapid characterization of particular biological parts and devices requires new methods as existing methods are inefficient and error prone, and require extensive time-consuming experiments.An alternative to current methods is the use ell-free systems for rapid characterization. Cell-free systems are cell extracts that contain all the machinery that allows biological parts to function and as such one can analyse many parts quickly without using living cells. This therefore speeds up the whole process. However, cell free systems can be variable and the results can be different between different researchers. The overall goal of this project is to further advance standardized cell-free systems using both computer models and new biochemical measurement tools. Such standardized systems will both explore the boundaries of cell-free prototyping and characterization, and enable more detailed understanding of key mechanisms, accelerating the usage and broader utility of cell-free systems in industry and academia.

合成生物学是一个新兴的工程学领域，旨在基于“自下而上”的方法重建复杂的生物分子系统，建立生物系统设计的系统框架。工程方法在设计中的应用很有吸引力，因为可以在生命系统中识别出许多工程相似之处。然而，生物系统非常复杂和动态，难以设计。特定生物部件和设备的快速表征需要新的方法，因为现有方法效率低下且容易出错，并且需要大量耗时的实验。当前方法的替代方法是使用无细胞系统进行快速表征。无细胞系统是一种细胞提取物，包含使生物部件发挥作用的所有机制，因此可以在不使用活细胞的情况下快速分析许多部件。因此，这加快了整个过程。然而，无细胞系统可能是可变的，并且不同研究人员之间的结果可能不同。该项目的总体目标是使用计算机模型和新的生化测量工具进一步推进标准化无细胞系统。这种标准化系统将探索无细胞原型制作和表征的边界，并能够更详细地理解关键机制，加速无细胞系统在工业界和学术界的使用和更广泛的应用。

项目成果

basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method.

• DOI: 10.1093/synbio/ysac023
• 发表时间: 2022
• 期刊: SYNTHETIC BIOLOGY
• 影响因子: 3.2
• 作者: Haines, Matthew C.;Carling, Benedict;Marshall, James;Shenshin, Vasily A.;Baldwin, Geoff S.;Freemont, Paul;Storch, Marko
• 通讯作者: Storch, Marko

DNA synthesis technologies to close the gene writing gap.

• DOI: 10.1038/s41570-022-00456-9
• 发表时间: 2023
• 期刊: NATURE REVIEWS CHEMISTRY
• 影响因子: 36.3
• 作者: Hoose, Alex;Vellacott, Richard;Storch, Marko;Freemont, Paul S;Ryadnov, Maxim G
• 通讯作者: Ryadnov, Maxim G

Cell-free gene expression

• DOI: 10.1038/s43586-021-00046-x
• 发表时间: 2021-07-15
• 期刊: NATURE REVIEWS METHODS PRIMERS
• 作者: Garenne, David;Haines, Matthew C.;Noireaux, Vincent
• 通讯作者: Noireaux, Vincent

Pandemic preparedness: synthetic biology and publicly funded biofoundries can rapidly accelerate response time.

• DOI: 10.1038/s41467-022-28103-3
• 发表时间: 2022-01-21
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Vickers CE;Freemont PS
• 通讯作者: Freemont PS

basicsynbio and the BASIC SEVA collection: Software and vectors for an established DNA assembly method

basicsynbio 和 BASIC SEVA 系列：用于已建立的 DNA 组装方法的软件和载体

• DOI: 10.1101/2022.01.06.446575
• 发表时间: 2022
• 作者: Haines M