EFFECTS OF ETHANOL ON GASTROINTESTINAL BIOCHEMISTRY AND PHYSIOLOGY

乙醇对胃肠生物化学和生理学的影响

• 批准号: 3821222
• 负责人: M-T HUANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3821222
• 关键词: alcohol dehydrogenase; carbohydrate biosynthesis; carbohydrate metabolism; detoxification; dietary carbohydrates; ethanol; gastrointestinal nutrient absorption; glucose metabolism; glycogen; glycogenesis; liver circulation; liver function; liver metabolism; nutrition related tag; oxidation reduction reaction; portal vein

A surgical method was developed to cannulate chronically both the portal and hepatic veins of laboratory rats. This experimental system is useful for studies on intestinal absorption and hepatic extractions of nutrients. With this experimental system, the following objectives can be determined (1) the effect of ethanol on GI absorption and liver metabolism and (2) glucose paradox. In the first study, the rate of ethanol elimination will be determined in rats meal-fed with diet containing glucose, fructose, mixture of glucose and fructose, and sucrose to determine the importance of alcohol dehydrogenase and redox state in liver on the metabolism of ethanol in vivo. In the second study, portal-hepatic difference of glucose and gluconeogenetic precursors will be determined in order to resolve the paradox that liver can not utilize glucose efficiently. Our results, in the latter subject, show that liver can utilize exogenons glucose and can synthesize glycogen directly from exogenous glucose directly. Recent theory on the pathway of glycogen synthesize in liver (Glucose-C3-G6P-Glucogen) was found to be based on questionable data and inadequate method of calculation.

一种手术方法被开发出来，用于慢性插管 实验大鼠的门静脉和肝静脉。这项实验 该系统可用于肠道吸收和肝脏的研究 营养素的提取。有了这个实验系统， 可以确定以下目标：(1)乙醇的效果 胃肠吸收与肝脏代谢的关系；(2)葡萄糖悖论。在……里面 第一项研究，将确定乙醇的消除速度 大鼠进食含有葡萄糖、果糖、 葡萄糖和果糖，以及蔗糖来确定重要性 乙醇脱氢酶与肝脏氧化还原状态对代谢的影响 体内的乙醇含量。在第二项研究中，门静脉-肝脏的差异 葡萄糖和糖异生前体的含量将在#年确定 为化解肝不能利用葡萄糖的悖论 效率很高。在后一项研究中，我们的研究结果表明，肝脏可以 利用外源葡萄糖可直接合成糖原 直接从外源葡萄糖中提取。关于这一途径的最新理论 肝糖原(葡萄糖-C3-G6P-糖原)的合成 被发现基于有问题的数据和不适当的方法 计算。