EFFECTS OF ETHANOL ON GASTROINTESTINAL BIOCHEMISTRY AND PHYSIOLOGY

乙醇对胃肠生物化学和生理学的影响

• 批准号: 3817377
• 负责人: M-T HUANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3817377
• 关键词: alcohol dehydrogenase; carbohydrate biosynthesis; carbohydrate metabolism; detoxification; dietary carbohydrates; ethanol; gastrointestinal nutrient absorption; glucose metabolism; glycogen; glycogenesis; laboratory rat; liver circulation; liver function; liver metabolism; nutrition related tag; oxidation reduction reaction; portal vein

A surgical method was developed to cannulate chronically both the portal and hepatic veins of laboratory rats. This experimental system is useful for studies on intestinal absorption and hepatic extractions of nutrients. With this experimental system, the following objectives can be determined (1) the effect of ethanol on Gl absorption and liver metabolism and (2) glucose paradox. In the first study, the rate of ethanol elimination will be determined in rats meal-fed with diet containing glucose, fructose, mixture of glucose and fructose, and sucrose to determine the importance of alcohol dehydrogenase and redox state in liver on the metabolism of ethanol in vivo. In the second study, portal-hepatic difference of glucose and gluconeogenetic precursors will be determined in order to resolve the paradox that liver can not utilize glucose efficiently. Our results, in the latter subject, show that liver can utilize exogenous glucose and can synthesize glycogen directly from exogenous glucose directly. Recent theory on the pathway of glycogen synthesize in liver (Glucose-C3-G6P-Glucogen) was found to be based on questionable data and inadequate method of calculation.

开发了一种手术方法， 实验大鼠的门静脉和肝静脉。 该实验 系统可用于肠吸收和肝吸收的研究 营养素的提取。 有了这个实验系统， 可以确定以下目标：（1）乙醇的影响 对葡萄糖吸收和肝脏代谢的影响;（2）葡萄糖反常。 在 在第一项研究中，将确定乙醇消除的速率， 在用含有葡萄糖、果糖、混合物 葡萄糖、果糖和蔗糖的含量来确定 乙醇脱氢酶和氧化还原状态对肝脏代谢的影响 乙醇在体内 在第二项研究中， 葡萄糖和葡萄糖生成前体的含量将在 为了解决肝脏不能利用葡萄糖的矛盾， 有效地我们的结果，在后一个主题中，表明肝脏 能利用外源性葡萄糖直接合成糖原， 直接从外源性葡萄糖中提取。 最近的理论的途径 发现肝糖原合成（葡萄糖-C3-G6 P-糖原 基于有问题的数据和不适当的方法， 计算.