PORTAL AND HEPATIC VEIN CANNULATION IN LABORATORY RAT

实验大鼠门静脉和肝静脉插管

• 批准号: 3822947
• 负责人: M-T HUANG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822947
• 关键词: alcohol dehydrogenase; detoxification; dietary carbohydrates; ethanol; gastrointestinal nutrient absorption; glucose metabolism; liver circulation; liver function; liver metabolism; nutrition related tag; oxidation reduction reaction; portal vein

A surgical method was developed to cannulate chronically both the portal and hepatic veins of laboratory rats. This experimental system is useful for studies on intestinal absorption and hepatic extractions of nutrients. With this experimental system, the following objectives can be determined (1) the effect of dietary carbohydrate on the elimination of ethanol and (2) glucose paradox. In the first study, the rate of ethanol elimination will be determined in rats meal-fed with diet containing glucose, fructose, mixture of glucose and fructose, and sucrose to determine the importance of alcohol dehydrogenase and redox state in liver on the metabolism of ethanol in vivo. In the second study, portal-hepatic difference of glucose and gluconeogenetic precursors will be determined in order to resolve the paradox that liver can not utilize glucose efficiently.

一种手术方法被开发出来， 和实验室大鼠的肝静脉。 这个实验系统是有用的 用于研究肠道吸收和肝脏提取营养物质。 利用该实验系统，可以确定以下目标 (1)饮食碳水化合物对消除乙醇的影响， (2)葡萄糖悖论 在第一项研究中， 将在用含有葡萄糖，果糖， 葡萄糖、果糖和蔗糖的混合物，以确定 乙醇脱氢酶和肝脏氧化还原状态对乙醇代谢的影响 in vivo. 在第二项研究中， 为了解决这一问题，将确定成矿前体。 肝脏不能有效利用葡萄糖的悖论。