MOLECULAR CLONING OF PYRUVATE DEHYDROGENASE GENE

丙酮酸脱氢酶基因的分子克隆

• 批准号: 3821233
• 负责人: B J SONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3821233
• 关键词: alcoholic beverage consumption; alcoholic hepatitis; alcoholism /alcohol abuse; butanols; diol; genetic mapping; genetic markers; liver cirrhosis; liver metabolism; liver toxic disorder; molecular cloning; molecular pathology; pyruvate dehydrogenase

Recent studies from our laboratory indicate that 2,3-butanediol, one of two unusual metabolites found in human alcoholic blood, is predominantly associated with human subjects who suffer alcoholic hepatitis or alcoholic cirrhosis (Casazza et al., Alcohol and Alcoholism, Suppl. 1: 607-609, 1987). The mechanism of the formation of 2,3-butanediol via pyruvate dehydrogenase was initially proposed by Veech et al. (Curr. Top. Cell. Regul 18: 151- 179, 1981). Based on the proposal, we have just started a project to clone pyruvate dehydrogenase (PDH) gene and to characterized its gene in light of developing a specific probe for a genetic marker for human alcoholism.

我们实验室最近的研究表明，2，3-丁二醇， 在人类酒精血液中发现的两种不寻常的代谢物之一， 主要与患有 酒精性肝炎或酒精性肝硬化（Casazza等人，醇 和Alcoholism，Suppl.1：607-609，1987）。 的机理 通过丙酮酸脱氢酶形成2，3-丁二醇， 最初由Veech等人（Curr. Top. Cell. 18：151- 179，1981）。 根据这个建议，我们刚刚开始了一个项目 目的：克隆丙酮酸脱氢酶（PDH）基因， 它的基因，根据开发一个遗传的特异性探针， 人类酒精中毒的标志