MOLECULAR CLONING OF PYRUVATE DEHYDROGENASE GENE

丙酮酸脱氢酶基因的分子克隆

• 批准号: 3817387
• 负责人: B J SONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3817387
• 关键词: alcoholic beverage consumption; alcoholic hepatitis; alcoholism /alcohol abuse; butanols; diol; genetic mapping; genetic markers; liver cirrhosis; liver metabolism; liver toxic disorder; molecular cloning; molecular pathology; nucleic acid probes; pyruvate dehydrogenase

Recent studies from our laboratory indicate that 2,3-butanediol, one of two unusual metabolites found in human alcoholic blood, is predominantly associated with human subjects who suffer alcoholic hepatitis or alcoholic cirrhosis (Casazza, et al., Alcohol and Alcoholism 1987;(suppl 1):607-609). Although the exact mechanism of 2,3-butanediol formation is not known, Veech and his associates have postulated that it could be generated by pyruvate dehydrogenase that are found in brain and testis (Curr Top Cell Regulation 1981;18:151-179). Based on the proposal, we have just started a project to clone genes encoding for pyruvate dehydrogenase complex using synthetic oligodexoynucleotide probes. By nucleotide sequencing, we have confirmed that our clones for ElX and EIB and E3 represent the authentic pyruvate dehydrogenase. Using these cDNA clones, we are studying the regulation of these genes especially in alcoholic subjects.

我们实验室最近的研究表明2,3-丁二醇，