MOLECULAR CLONING OF PYRUVATE DEHYDROGENASE GENE

我们实验室最近的研究表明，2，3-丁二醇， 在人类酒精血液中发现的两种不寻常的代谢物之一是 主要与酗酒的人类受试者有关 肝炎或酒精性肝硬变(Casazza等人，酒精和 酒精中毒1987；(附录1)：607-609)。尽管确切的机制 2，3-丁二醇的形成尚不清楚，Veech和他的同事们 推测它可能是由丙酮酸产生的 在大脑和睾丸中发现的脱氢酶(Curr Top Cell 条例1981；18：151-179)。根据这项建议，我们刚刚 启动了一项克隆丙酮酸编码基因的项目 使用人工合成的寡核苷酸探针的脱氢酶复合体。 通过核苷酸测序，我们已经确认我们的ELX克隆 EIB和E3代表真正的丙酮酸脱氢酶。 利用这些cdna克隆，我们正在研究这些调控。 尤其是在酗酒的受试者身上。

Recent studies from our laboratory indicate that 2,3-butanediol, one of two unusual metabolites found in human alcoholic blood, is predominantly associated with human subjects who suffer alcoholic hepatitis or alcoholic cirrhosis (Casazza, et al., Alcohol and Alcoholism 1987;(suppl 1):607-609). Although the exact mechanism of 2,3-butanediol formation is not known, Veech and his associates have postulated that it could be generated by pyruvate dehydrogenase that are found in brain and testis (Curr Top Cell Regulation 1981;18:151-179). Based on the proposal, we have just started a project to clone genes encoding for pyruvate dehydrogenase complex using synthetic oligodexoynucleotide probes. By nucleotide sequencing, we have confirmed that our clones for ElX and EIB and E3 represent the authentic pyruvate dehydrogenase. Using these cDNA clones, we are studying the regulation of these genes especially in alcoholic subjects.