ROLE OF PDGF EXPRESSSION IN THE NEOPLASTIC PROCESS

PDGF 表达在肿瘤形成过程中的作用

• 批准号: 3896371
• 负责人: M PECH
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3896371
• 关键词: fibrosarcoma; gene expression; genetic manipulation; genetic promoter element; genetic transcription; genetically modified animals; glioblastoma multiforme; growth factor receptors; human tissue; laboratory mouse; neoplasm /cancer genetics; neoplastic cell; neurons; oncogenes; oncoproteins; platelet derived growth factor; sarcoma; simian virus; transposon /insertion element; viral carcinogenesis

The v-sis oncogene of simian sarcoma virus (SSV) encodes a protein which is closely related to the human PDGF-B or -2 chain. The c-sis/platelet-derived growth factor (PDGF)-B gene. The human homologue for v-sis. has been cloned and characterized. When this gene is expressed under the control of a retrovirus promoter and introduced in NIH 3T3 cells in culture, it confers the transformed phenotype to these cells. The PDGF-B chain transcript has been observed in a variety of human glioblastomas and fibrosarcomas. In contrast, this transcript is not detectable in normal glial cells or fibroblasts. All of these findings along with the immunological demonstration of the PDGF-B product expressed by such human tumor cells support a role of PDGF-B in tumorigenesis. We have investigated the regulation of expression of this growth factor in vivo and in vitro. Overexpression of PDGF-B in vivo leads to formation of fibrosarcomas in mice. Low levels of expression of this human growth factor in different tissues was also achieved by introducing the gene into the germline of transgenic mice.

猿猴肉瘤病毒（SSV）的v-sis癌基因编码一种蛋白质， 与人PDGF-B或-2链密切相关。c-sis/血小板源性 PDGF-B基因。 v-sis的人类同源物。已经 克隆并鉴定。当这个基因在 一种逆转录病毒启动子并导入培养的NIH 3 T3细胞中， 赋予这些细胞转化的表型。PDGF-B链 已在多种人类胶质母细胞瘤中观察到转录物， 纤维肉瘤相比之下，这种转录本在正常人中检测不到。 神经胶质细胞或成纤维细胞。所有这些发现沿着 由这种人表达的PDGF-B产物的免疫学证明 肿瘤细胞支持PDGF-B在肿瘤发生中的作用。我们已经调查 该生长因子在体内和体外表达的调节。 PDGF-B在体内的过度表达导致纤维肉瘤的形成， 小鼠这种人类生长因子的低水平表达， 组织也是通过将基因导入到 转基因小鼠