MOLECULAR CLONING AND CHARACTERIZATION OF RETROVIRUSES ASSOCIATED WITH AIDS

艾滋病的一个突出特点是HIV-1逆转录病毒之间的异质性 基因组分子克隆，限制性内切酶分析，核苷酸 测序和感染性研究已被用于表征 各种隔离物。这些研究提供了有关他们的信息。 结构和地理多样性，以及可能的程度， 不同HIV-1分离株之间的抗原变异性和致病性。 从不同分子克隆的HIV分离株制备的病毒是 目前用于制备单克隆抗体以测试它们的 对广谱HIV分离株的中和活性。 来自感染HIV-1（LAV）的淋巴细胞的T细胞克隆（ACH-2）产生 HIV-1对佛波醇肉豆蔻乙酸酯（PMA）刺激的反应。一个 分离物被分子克隆，并在转染到T细胞中后， 生产病毒;然而，只有在PMA的存在下。传代至新鲜 通过逆转录酶活性监测淋巴细胞产生病毒 (RT)在没有诱导PMA的情况下。混配实验 在ACH-2克隆和LAV亲本之间交换gag-pol-env区 克隆，恢复ACH-2对野生型LAV的生物活性。ACH-2 分离物的致病性可能提供了一个了解的机制， HM，因为它涉及慢性感染和/或潜伏期和病毒诱导。

A prominent feature of AIDS is the heterogeneity among HIV-1 retroviral genomes. Molecular cloning, restriction enzyme analysis, nucleotide sequencing, and infectivity studies have been used to characterized the various isolates. These studies provide information regarding their structural as well as geographic diversity and, possibly, the degree of antigenic variability and pathogenicity among different HIV-1 isolates. Virus prepared from the different molecular cloned HIV isolates are presently being used to prepare monoclonal antibodies to test their neutralization activity against a wide spectrum of HIV isolates. A T cell clone (ACH-2) from lymphocytes infected with HIV-1 (LAV) produced HIV-1 in response to stimulation with phorbol myristic acetate (PMA). An isolate was molecularly cloned and, upon transfection into T cells, produced virus; however, only in the presence of PMA. Passage onto fresh lymphocytes produced virus as monitored by reverse transcriptase activity (RT) in the absence of the inducing PMA. Mixing and matching experiments swapping the gag-pol-env region between the ACH-2 clone and LAV parent clone, restored biological activity of ACH-2 to wild-type LAV. The ACH-2 isolate may provide an understanding of the mechanism of pathogenicity of HM as it relates to chronic infections and/or latency and viral induction.