MOLECULAR CLONING AND CHARACTERIZATION OF RETROVIRUSES ASSOCIATED WITH AIDS

艾滋病逆转录病毒基因组的异质性是 慢病毒，分子克隆，限制性内切酶分析，核苷酸 测序和聚合酶链式反应技术用于 人类免疫缺陷病毒1型(HIV-1)分离株的特征。这个 目的是获得有关结构和结构的信息 艾滋病毒在生化、致病和抗原性方面的多样性 可变性。我们克隆的大多数HIV分离株都是嗜T细胞。我们有 成功地从向巨噬细胞中获得了一个完整的分子克隆 病毒分离。尽管T细胞是HIV复制的主要目标 在外周血液中，巨噬细胞是感染艾滋病毒的主要细胞。 大多数组织中的细胞类型。巨噬细胞可能是主要的储存库。 感染艾滋病毒，并在个人身上持续感染多年。 一个单一的HIV巨噬细胞趋化分离株被分子克隆，并在 转染HeLa细胞后与外周血淋巴细胞共培养 巨噬细胞，产生病毒颗粒(RT)。产生的病毒是 具有传染性，可在外周血淋巴细胞或巨噬细胞中复制。确实是 不能在连续的T细胞系(CEM)中复制。增长研究和 对该分离物的生化分析正在进行中。

Heterogeneity among AIDS retroviral genomes is a distinct feature of lentiviruses, Molecular cloning, restriction enzyme analysis, nucleotide sequencing, and polymerase chain reaction techniques were used to characterize isolates of Human Immunodeficiency Virus Type 1 (HIV-1). The objective was to obtain information pertaining to the structure and diversity of HIV with respect to its biochemical, pathogenic, and antigenic variability. Most HIV isolates we have cloned are T-cell tropic. We have succeeded in obtaining a complete molecular clone from a macrophagetropic viral isolate. Although T-cells are the major target for HIV replication in peripheral blood, macrophages represent the predominant HIV-infected cell type in most tissues. Macrophages are probably the primary reservoir of HIV, and sustain a persistent infection in individuals for many years. A single HIV macrophagetropic isolate was molecularly cloned, and upon transfection into HeLa cells followed by cocultivation on PBLs or macrophages, produced viral particles (RT). The virus produced was infectious and could replicate in either PBLs or macrophages. It was unable to replicate in a continuous T-cell line (CEM). Growth studies and biochemical analysis with this isolate are in progress.