MOLECULAR CLONING AND CHARACTERIZATION OF HUMAN RETROVIRUSES

艾滋病逆转录病毒基因组之间的异质性是艾滋病病毒的一个显著特征， 慢病毒，分子克隆，限制性内切酶分析，核苷酸 测序和聚合酶链反应技术被用来 鉴定人类免疫缺陷病毒1型（HIV-1）分离株。 的 目的是获得有关该结构的信息， HIV在生化、致病性和抗原性方面的多样性 可变性 我们克隆的大多数HIV分离株都是嗜T细胞的。 我们有 成功地从巨噬细胞中获得了一个完整的分子克隆 病毒分离物。 虽然T细胞是HIV复制的主要目标， 在外周血中，巨噬细胞代表了主要的HIV感染者， 大多数组织中的细胞类型。 大型浮游生物可能是 艾滋病病毒，并持续多年的个人感染。 对单个HIV巨噬细胞分离株进行分子克隆， 转染到HeLa细胞中，然后在PBL上共培养，或 巨噬细胞产生病毒颗粒（RT）。 产生的病毒是 感染性，并可在PBL或巨噬细胞中复制。 这是 不能在连续T细胞系（CEM）中复制。 生长研究和 对该分离株的生化分析正在进行中。

Heterogeneity among AIDS retroviral genomes is a distinct feature of lentiviruses, Molecular cloning, restriction enzyme analysis, nucleotide sequencing, and polymerase chain reaction techniques were used to characterize isolates of Human Immunodeficiency Virus Type 1 (HIV-1). The objective was to obtain information pertaining to the structure and diversity of HIV with respect to its biochemical, pathogenic, and antigenic variability. Most HIV isolates we have cloned are T-cell tropic. We have succeeded in obtaining a complete molecular clone from a macrophagetropic viral isolate. Although T-cells are the major target for HIV replication in peripheral blood, macrophages represent the predominant HIV-infected cell type in most tissues. Macrophages are probably the primary reservoir of HIV, and sustain a persistent infection in individuals for many years. A single HIV macrophagetropic isolate was molecularly cloned, and upon transfection into HeLa cells followed by cocultivation on PBLs or macrophages, produced viral particles (RT). The virus produced was infectious and could replicate in either PBLs or macrophages. It was unable to replicate in a continuous T-cell line (CEM). Growth studies and biochemical analysis with this isolate are in progress.