SYSTEM SOFTWARE FOR PROTEIN AND NUCLEIC ACID STRUCTURE ANALYSIS

这部分程序的目的是使 一般生物医学界的各种显微镜控制和 图像采集/分析程序， 科. 该计划是基于定量显微镜具有 作为最低系统PC/AT（或完全兼容的 MS-DOS机器），最小内存为512 Kb， 30兆硬盘。 所有与显微镜的最终接口 将通过商用接口卡实现 其具有适当的模拟和数字信号I/O。 图像 采集硬件也将是商用的。 我们计划 在公共领域放置一系列源程序， 在C中（在PC世界中实现最大的可移植性），这将允许 越来越多的变化和复杂的功能，以执行 在计算机显微控制下获得的图像。 的程序 用户可以使用的将取决于有多少额外的 他所拥有的资源超过了所提到的最低限度。 功能上 例如，控制程序同样适用于 倒置显微镜（打开组织培养物， 受控图像采集）。 自由的规定是在 用于控制附加步进电机功能的软件 其可以是用户定义的（例如，聚光器焦点、液体流动 进出腔室等）。 计划包括 交互式图像处理的可能性，采用PC 兼容的高分辨率显示器，具有灰度级能力。 此选项和其他选项可作为用户兴趣的函数 以及特定PC或PC上可用的资源级别 有问题。

The object of this part of the program is to make available to the general biomedical community the various microscope control and image acquisition/ analysis procedures developed within the section. The plan is based on the quantitative microscopist having available to him as a minimum system a PC/AT (or a fully compatible MS-DOS machine) with a minimum of 512 Kb of memory and a standard 30 megabyte hard disk. All final interfacing with the microscope will be accomplished via commercially available interface cards which have the appropriate analog and digital signal I/O. Image acquisition hardware will also be of the commercial type. We plan to place in the public domain a series of source programs, written in C (for maximum portability in the PC world) which will allow incrementally more varied and complex functions to be performed on images acquired under computer microscopic control. The procedures which the user can employ will depend upon how much additional resources he has available beyond the noted minimum. Functionally speaking, the programs for control, will for example, apply equally well to inverted microscopy (opening up tissue cultures to controlled image acquisition). Liberal provision is being made in the software for control of additional stepping motor functions which could be user defined (e.g. condenser focus, flow of liquids into and out of a chamber, etc). It is planned to include the possibility for interactive image processing, employing a PC compatible high resolution display with gray level capability. This and other options are available as a function of user interest and the level of resources available on the particular PC or PCs in question.