SYSTEM SOFTWARE FOR PROTEIN AND NUCLEIC ACID STRUCTURE ANALYSIS

该计划的这一部分的目的是使 一般生物医学界的各种显微镜 制定控制和图像采集/分析程序 在该部分。 该计划是基于量化的 作为一个最低限度的系统， PC/AT（或完全兼容的MS-DOS机器）， 512 Kb的内存和一个标准的20兆字节的硬盘。 所有 与显微镜的接口将通过 商用接口卡， 适当的模拟和数字信号I/O。 图像采集 硬件也将是商业类型的。 我们计划把 在公共领域的一系列源程序，用C (for最大的便携性在PC世界），这将允许 越来越多样化和复杂的功能， 在计算机显微镜下获得的图像上进行 控制 用户可以使用的程序将取决于 他有多少额外的资源， 以上提到的最低。 从功能上讲， 控制，例如，将同样适用于倒置 显微镜（打开组织培养物以控制图像 收购）。 在软件中， 控制附加的步进电机功能， 用户定义（如聚光器焦点、液体流入和流出 室等）。 计划包括以下可能性： 交互式图像处理，采用PC兼容的高 分辨率显示，具有一定的灰度级能力。 这个和 其他选项可作为用户兴趣的函数而使用， 他的PC上的可用资源水平。

The object of this part of the program is to make available to the general biomedical community the various microscope control and image acquisition/analysis procedures developed within the section. The plan is based on the quantitative microscopist having available to him as a minimum system a PC/AT (or a fully compatible MS-DOS machine) with a minimum of 512 Kb of memory and a standard 20 megabyte hard disk. All interfacing with the microscope will be accomplished via commercially available interface cards which have the appropriate analog and digital signal I/O. Image acquisition hardware will also be of the commercial type. We plan to place in the public domain a series of source programs, written in C (for maximum portability in the PC world) which will allow incrementally more varied and complex functions to be performed on images acquired under computer microscopic control. The procedures which the user can employ will depend upon how much additional resources he has available beyond the above noted minimum. Functionally speaking, the programs for control, will for example, apply equally well to inverted microscopy (opening up tissue cultures to controlled image acquisition). Liberal provision is being made in the software for control of additional stepping motor functions which could be user defined (e.g. condenser focus, flow of liquids into and out of a chamber, etc). It is planned to include the possibility for interactive image processing, employing a PC compatible high resolution display, with some gray level capability. This and other options are available as a function of user interest and the level of resources available on his PC.