SYSTEM SOFTWARE FOR PROTEIN AND NUCLEIC ACID STRUCTURE ANALYSIS

该计划的这一部分的目的是使 普通生物医学界的各种显微镜 制定了控制和图像采集/分析程序 在这一节内。该计划是以量化为基础的 显微镜检验员至少有一套系统可用 PC/AT(或完全兼容的MS-DOS机器)至少 512KB的内存和一个标准的20MB硬盘。全 与显微镜的接口将通过以下方式完成 市面上出售的接口卡具有 适当的模拟和数字信号I/O图像采集 硬件也将是商业型的。我们计划将 在公共领域中，用C编写的一系列源程序 (在PC世界中实现最大的便携性)，这将允许 将逐步实现更多样和更复杂的功能 在计算机显微镜下获取的图像上执行 控制力。用户可以采用的程序将取决于 根据他有多少可用的额外资源 以上提到的最低要求。从功能上讲， 例如，控件也同样适用于反转 显微镜(将组织培养向受控图像开放 收购)。软件中为以下方面做了自由的规定 控制额外的步进电机功能，这些功能可以 用户定义(例如冷凝器焦点、液体流入和流出 (房间等)。计划包括以下可能性： 交互式图像处理，采用PC兼容的HIGH 分辨率显示，具有一定的灰度级能力。这和 其他选项可根据用户的兴趣和 他的PC上可用的资源级别。

The object of this part of the program is to make available to the general biomedical community the various microscope control and image acquisition/analysis procedures developed within the section. The plan is based on the quantitative microscopist having available to him as a minimum system a PC/AT (or a fully compatible MS-DOS machine) with a minimum of 512 Kb of memory and a standard 20 megabyte hard disk. All interfacing with the microscope will be accomplished via commercially available interface cards which have the appropriate analog and digital signal I/O. Image acquisition hardware will also be of the commercial type. We plan to place in the public domain a series of source programs, written in C (for maximum portability in the PC world) which will allow incrementally more varied and complex functions to be performed on images acquired under computer microscopic control. The procedures which the user can employ will depend upon how much additional resources he has available beyond the above noted minimum. Functionally speaking, the programs for control, will for example, apply equally well to inverted microscopy (opening up tissue cultures to controlled image acquisition). Liberal provision is being made in the software for control of additional stepping motor functions which could be user defined (e.g. condenser focus, flow of liquids into and out of a chamber, etc). It is planned to include the possibility for interactive image processing, employing a PC compatible high resolution display, with some gray level capability. This and other options are available as a function of user interest and the level of resources available on his PC.