ANALYSIS AND SYNTHESIS OF NUCLEIC ACID SECONDARY STRUCTURE

核酸二级结构的分析与合成

• 批准号: 4691713
• 负责人: L L LIPKIN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691713
• 关键词: computer simulation; conformation; electron microscopy; mathematical model; nucleic acid denaturation; nucleic acid structure; thermodynamics

Research has continued in collaboration with Dr. Ruth Nussinov from the School of Medicine at Tel Aviv University in the area of B-DNA distortions based upon the Calladine-Dickerson rules. Two extendable systems have been developed and enhanced which incorporate the four rules of distortion, namely helical twist, base pair roll, and torsion and propeller twist. These systems have the facility to search for patterns that are not known to exist a priori. Feature enhancement and feature detection techniques are available to aid the user in an interactive environmental to discover new patterns of potential interset. These techniques have indicated the existence of morphologic structures which appear to be correlated to functionality within the molecule. Work has also begun on a new algorithm to measure similarity among secondary strucutes of RNA molecules. This technique when perfected should permit the determination of multiple levels of similarity amongst several molecules of the same or different classes. Also, the RNA secondary structure drawing programs has been sent to several more institutions around the world. It is proving to be quite useful in depicting the structures of RNA molecules after they have been folded.

研究继续在与博士露丝Nussinov从合作 特拉维夫大学医学院B-DNA畸变领域 根据卡拉丁-迪克森法则 两个可扩展的系统已被 发展和加强了这四个规则的扭曲， 即螺旋扭曲、基本对滚转、以及扭转和螺旋桨扭曲。 这些系统具有搜索未知模式的功能 存在于先验之中。 特征增强和特征检测技术 可用于帮助用户在交互式环境中发现 新的潜在干扰模式。 这些技术表明， 形态结构的存在似乎与 分子内的功能。 一种新算法的研究也已经开始 来测量RNA分子二级结构之间的相似性。 这 技术完善后，应允许多层次的测定 相同或不同类别的几个分子之间的相似性。 此外，RNA二级结构绘图程序已发送到几个 世界各地的更多机构。 它被证明是非常有用的， 描绘了RNA分子折叠后的结构。