TYROSINE PHOSPHATASES AND INSULIN RESISTANCE IN THE AGED

靶细胞的胰岛素抵抗在非胰岛素依赖性 老年糖尿病。 我们一直在研究新的方法 为了鉴定和表征胰岛素受体磷酸酶， 试图了解这种磷酸酶活性是如何在 作为治疗的靶点。 我们已经证明了我们最近引入的透化细胞模型 提供了独特的机会来研究 胰岛素受体的磷酸化和去磷酸化， 相关的膜结合蛋白酪氨酸磷酸酶。 是 表明细胞内的区室化和与细胞的相互作用 其它推定的调节蛋白得以保留。而且这 该系统允许研究正常和突变胰岛素的活性 受体的以及合成肽对免疫系统的影响 受体的磷酸化。

Insulin resistance at the target cell is common in non-insulin dependent diabetes mellitus of the elderly. We have been investigating novel ways to identify and characterize a putative insulin receptor phosphatase in an attempt to understand how this phosphatase activity can be regulated in vivo as a target for treatment. We have demonstrated that our recently introduced permeabilized cell model offers unique opportunities to investigate the intimate regulation of insulin receptor phosphorylation and dephosphorylation by closely- associated membrane-bound protein tyrosine phosphatase(s). It is suggested that compartmentalization within the cell and interactions with other putative regulatory proteins are maintained. Furthermore, this system has allowed the study of the activity of normal and mutant insulin receptor. and the effect of synthetic peptides on the extent and pattern of receptor phosphorylation.