CALCIUM-ACTIVATED NEUTRAL PROTEINASE IN CNS

中枢神经系统中钙激活的中性蛋白酶

• 批准号: 3402434
• 负责人: NAREN L BANIK
• 金额: $ 6.84万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-02-01 至 1989-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3402434
• 关键词: axon; calcium; cell type; central nervous system; developmental neurobiology; electrophoresis; glia; histochemistry /cytochemistry; immunochemistry; isozymes; laboratory rabbit; laboratory rat; myelin; organelles; peptidases; peripheral nervous system; radioassay

This project explores the hypothesis that a calcium-activated neutral proteinase(s) (CANP) is associated with myelin and/or localized in axons and glial cells. This thesis has been developed from our demonstration of a calcium-mediated degradation of cytoskeletal as well as myelin proteins by a proteinase(s) present in spinal cord and brain white matter. Thus our detailed studies will be 1) to establish that CANP is associated with myelin: we plan to examine the enzyme (CANP) activity in different regions of brain, in myelin and subcellular fractions of brain during development and myelin from CNS and PNS of different species, and 2) to examine the localization of CANP by immunocytochemical technique after antisera is raised from purified CANP (which we have recently purified from bovine brain). The presence of this enzyme in myelin and related structures or cells may play a crucial role in the breakdown of myelin in demyelinating diseases (e.g. MS).

这个项目探索了一种假设，即钙激活的中性 蛋白水解酶(S)(CANP)与髓鞘和/或定位于轴突 和神经胶质细胞。本论文是从我们的演示中发展而来的 钙介导的细胞骨架和髓鞘蛋白的降解 由脊髓和脑白质中存在的一种蛋白酶(S)所致。因此，我们的 将进行详细的研究1)以确定CANP与 髓鞘：我们计划检测不同区域的酶(CANP)活性 在发育过程中，大脑的髓鞘和亚细胞部分 和来自不同物种的中枢神经系统和三叉神经节的髓鞘，以及2)检查 抗血清免疫细胞化学技术定位CANP的研究 来自纯化的CANP(我们最近从牛身上提纯 大脑)。这种酶在髓鞘和相关结构中的存在或 细胞可能在脱髓鞘过程中髓鞘的分解中起关键作用 疾病(如多发性硬化症)。

项目成果

Effects of detergents on Ca(2+)-activated neural proteinase activity (calpain) in neural and non-neural tissue: a comparative study.

去污剂对神经和非神经组织中 Ca(2) 激活的神经蛋白酶活性（钙蛋白酶）的影响：一项比较研究。

• DOI: 10.1007/bf00969015
• 发表时间: 1992
• 期刊: Neurochemical research
• 影响因子: 4.4
• 作者: Banik,NL;Chakrabarti,AK;Hogan,EL
• 通讯作者: Hogan,EL

Ca2+-mediated degradation of central nervous system (CNS) proteins: topographic and species variation.

Ca2 介导的中枢神经系统 (CNS) 蛋白降解：地形和物种变异。

• DOI: 10.1007/bf00999722
• 发表时间: 1987
• 期刊: Metabolic brain disease
• 影响因子: 3.6
• 作者: Banik,NL;Happel,RD;Sostek,MB;Chiu,FC;Hogan,EL
• 通讯作者: Hogan,EL

Pathogenesis of myelin breakdown in demyelinating diseases: role of proteolytic enzymes.

脱髓鞘疾病中髓磷脂分解的发病机制：蛋白水解酶的作用。

• 发表时间: 1992
• 期刊: Critical reviews in neurobiology
• 作者: Banik,NL

Calcium-activated neutral proteinase (CANP; calpain) activity in Schwann cells: immunofluorescence localization and compartmentation of mu- and mCANP.

雪旺细胞中钙激活的中性蛋白酶（CANP；钙蛋白酶）活性：免疫荧光定位和 mu- 和 mCANP 的划分。

• DOI: 10.1002/jnr.490290310
• 发表时间: 1991
• 期刊: Journal of neuroscience research
• 影响因子: 4.2
• 作者: Banik,NL;DeVries,GH;Neuberger,T;Russell,T;Chakrabarti,AK;Hogan,EL
• 通讯作者: Hogan,EL

Differential regulation of myelin gene expression in SV40 T antigen-transfected rat glioma C6 cells.

SV40 T 抗原转染的大鼠神经胶质瘤 C6 细胞中髓磷脂基因表达的差异调节。

• DOI: 10.1007/bf01000381
• 发表时间: 1991
• 期刊: Metabolic brain disease
• 影响因子: 3.6
• 作者: Bong,M;Chakrabarti,A;Banik,N;Hogan,EL;Kanoh,M;Wiggins,RC;Konat,G
• 通讯作者: Konat,G