Transformative Imaging for Quantitative Biology (TIQBio) Partnership

定量生物学变革成像 (TIQBio) 合作伙伴关系

• 批准号: EP/V038036/1
• 负责人: Sumeet Mahajan
• 金额: $ 219.61万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV038036%2F1
• 关键词: Transformative; Imaging; Quantitative; Biology; TIQBio

The Transformative Imaging for Quantitative Biology (TIQBio) partnership aims to develop disruptive technology for the benefit of UK Plc and for solving problems in industry and academia. The mainstay of current imaging methods to look at pre-clinical biological samples is fluorescence microscopy. This technique relies on the use of tags, which emit light when illuminated by the microscope, allowing the location of the structures or molecules to which they are attached to be determined. Insertion or attachment of a tag is an invasive process for any living system and can alter its behaviour and the way it functions. Furthermore, all living systems, tissue and cells are inherently 3-dimensional. Therefore to image in 3D one has to point-by-point collect fluorescence signal and reconstruct an image. This is a very slow and damaging process especially for 3-D live samples that represent real-life conditions. For discovering new drugs or for studying mechanisms in diseases or healing it is obvious that one should use conditions that are as near to real life as possible, before human testing. This is why most biomedical researchers and industrial sectors that operate in the area of diseases, drugs or therapeutics want to use life-like samples. At the moment however, the tools to image them in 3D and in an unperturbed, non-damaging manner simply do not exist. Furthermore, it is desirable to work at the highest resolution so we can see the smallest things that exist at the nanoscale in such biological systems and obtain holistic information about the chemical composition and structural order. This information will reveal unprecedented insight and hence help understand diseases or why a particular drug candidate does or does not work allowing better ones to be made. TIQBio will address these challenges so that unperturbed, live imaging can be carried out at an unprecedented resolution level in full 3D, with holistic information from multiple readouts carried out rapidly on 100s of test biological models. These innovative tools and technologies will allow the discovery of drugs to be improved, reduce costs for bringing a drug to market benefiting the pharma industry and patients alike. Patients with rare diseases or in lower income countries may gain access to new drugs because of the proposed disruptive technology. Biomedical researchers will benefit as they will be able to understand phenomena without misleading results due to tags; the use of real life-like models will better inform or protect the public through the development of therapies or defence countermeasures.

定量生物学变革成像（TIQBio）合作伙伴关系旨在开发颠覆性技术，以造福英国公司，并解决工业和学术界的问题。当前用于观察临床前生物样品的成像方法的主要是荧光显微镜。这种技术依赖于标签的使用，当被显微镜照射时，标签会发光，从而可以确定它们所连接的结构或分子的位置。标签的插入或附着对任何生命系统来说都是一个侵入性的过程，可以改变其行为和功能。此外，所有生命系统、组织和细胞本质上都是三维的。因此，为了在3D中成像，必须逐点收集荧光信号并重建图像。这是一个非常缓慢和破坏性的过程，特别是对于代表现实生活条件的3D活体样本。为了发现新药或研究疾病或治疗的机制，在人体试验之前，显然应该使用尽可能接近真实的生活的条件。这就是为什么大多数生物医学研究人员和在疾病、药物或治疗领域工作的工业部门都希望使用类似生命的样品。然而，目前还不存在以不受干扰、无损伤的方式对它们进行3D成像的工具。此外，希望以最高分辨率工作，以便我们可以看到存在于这种生物系统中的纳米级的最小事物，并获得有关化学组成和结构顺序的整体信息。这些信息将揭示前所未有的洞察力，从而有助于了解疾病或为什么特定的候选药物有效或无效，从而可以制造出更好的药物。TIQBio将应对这些挑战，以便能够以前所未有的全3D分辨率水平进行不受干扰的实时成像，并在数百个测试生物模型上快速进行多个读数的整体信息。这些创新的工具和技术将使药物的发现得到改善，降低将药物推向市场的成本，使制药行业和患者受益。由于拟议的颠覆性技术，患有罕见疾病或低收入国家的患者可能会获得新药。生物医学研究人员将从中受益，因为他们将能够理解现象，而不会因标签而产生误导性结果;使用真实的类生命模型将通过开发治疗或防御对策更好地告知或保护公众。

项目成果

1840-nm femtosecond thulium fiber laser system for label-free third-harmonic generation microscopy

用于无标记三次谐波显微镜的 1840 nm 飞秒铥光纤激光系统

• DOI: 10.1364/cleo_at.2022.jm4e.5
• 发表时间: 2022
• 作者: Xu L

From Mars to humans: interactive Raman spectroscopy-based outreach activities

• DOI: 10.1117/12.2646462
• 发表时间: 2022-09
• 作者: Jacob Kleboe;Helen Szoor-McElhinney;Hiroki Cook;S. Lane;Niall Hanrahan;James Read;Tommy Loan;S. Mahajan

15-µJ picosecond hollow-core-fiber-feedback optical parametric oscillator.

15 µJ 皮秒空芯光纤反馈光学参量振荡器。

• DOI: 10.1364/oe.494037
• 发表时间: 2023
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Wu Y

Harnessing Raman spectroscopy and Multimodal Imaging of Cartilage for Osteoarthritis Diagnosis

利用拉曼光谱和软骨多模态成像进行骨关节炎诊断

• DOI: 10.1101/2023.09.05.23294936
• 发表时间: 2023
• 作者: Crisford A

Deep tissue imaging with multiphoton microscopy in the short-wavelength infrared windows

在短波长红外窗口中使用多光子显微镜进行深层组织成像

• DOI: 10.1117/12.2647553
• 发表时间: 2023
• 作者: Bourdakos K