DEVELOPMENT OF DEFINED MEDIUM FOR EMBRYO CELLS

这个项目的主要目的是开发一种无血清的培养液 适合原代和早期传代的SHE细胞生长。 提供这样一种媒介将有助于标准化 遗传毒性检测方案和致癌机制研究， 与监管考虑相关的重要领域和 公共卫生。第一阶段研究包括： (A)通过不同种类的克隆滴定选择合适的基本培养基 在两种或两种以上候选基础上培养的血清批次。 (B)用生长因子和激素替代血清。优化 通过克隆滴定测定成分和浓度。 (C)测定SHE细胞在无血清培养条件下的克隆生长反应。 (D)血清中SHE细胞分泌转化生长因子的测定- 通过诱导NRK细胞的贴壁非贴壁生长的自由培养液，以及 单个转化生长因子β1和转化生长因子β2的鉴定。 (她=叙利亚仓鼠胚胎)

The primary purpose of this project is to develop a serum-free medium suitable for the growth of primary and early passage SHE cells. Availability of such a medium will facilitate standardization of geneotoxic testing protocols and studies on mechanisms of carcinogenesis, both significant areas relevant to regulatory considerations and the public health. Phase I studies involve: (a) Selection of a suitable basal medium by clonal titration of different serum lots in two or more candidate basal media. (b) Substitution of growth factors and hormones for serum. Optimization of components and concentrations by clonal titration. (c) Determine clonal growth response of SHE cells in serum-free medium. (d) Assay for transforming growth factor secretion by SHE cells in serum- free medium by induction of anchorage independent growth of NRK cells, and identification of individual TGF-beta 1 and TGF-beta 2. (SHE = Syrian Hamster Embryo)