STUDIES OF HUMAN ENDOTHELIAL CELLS OF DIVERSE ORIGIN

目的是研究培养的人的生长和功能 内皮细胞（EC）分离自不同的血管部位， 两种性别的老年捐赠者。 重点将放在审查 增殖潜力，选定的代谢活动，某些运输 功能和与止血功能相关的膜性质。 将从脑死亡的不同部位获得血管组织， 心脏跳动尸体，大血管和毛细血管EC将 与世隔绝 将生成原代、次级和克隆EC培养物， 分配到各个项目。 将追求以下目标： EC;检查EC的细胞遗传学特性;研究 各种文化成分之间的合作互动，因为它们影响 在来自年轻供体的II期和III期细胞中的人EC生长和 II型细胞来自老年供体;测量花生四烯酸的代谢产物 由人EC合成;表征和量化人EC相互作用 与因子VIII相关抗原和其他凝血组分;和 测量人EC中的囊泡摄取能力和胰岛素结合， 检查不同胶原基质对这些EC功能的影响。

The objective is to study the growth and function of cultured human endothelial cells (EC) isolated from diverse vascular sites in different aged donors of both sexes. Emphasis will be placed on examining proliferative potential, selected metabolic activities, certain transport functions, and membrane properties related to hemostatic function. Vascular tissue will be obtained from different sites in brain-dead, heart-beating cadavers, and large vessel and capillary EC will be isolated. Primary, secondary and cloned EC cultures will be generated and distributed to the component projects. The following goals will be pursued: determine the in vitro lifespan of EC; examine cytogenetic properties of EC; investigate the roles of and cooperative interactions between various culture components as they affect human EC growth in Phase II and Phase III cells from young donors and Phase II cells from older donors; measure the metabolites of arachidonic acid synthesized by human EC; characterize and quantify human EC interaction with Factor VIII-related antigen and other coagulation components; and measure vesicular ingestion capacity and insulin binding in human EC and examine the effect of different collagen substrata on these EC functions.