PRECLINICAL EVALUATION OF INTERMEDIATE ENDPOINTS

紫外线对SKH无毛小鼠皮肤癌发生的抑制作用 化学预防剂的筛选和分级。 该项目测量了 抑制紫外线诱导的SKH无毛小鼠皮肤癌发生， 筛选和排序化学预防剂。 这些研究利用计算机- 辅助定量图像分析（CIA）来测量广泛的 特殊的染色质纹理特征。 SKH无毛小鼠，6-8周 年龄和体重18-22克，正在使用。 对照组被 暴露于自然的12小时/黑暗，12小时/光照周期的窗口过滤 阳光 所有剩余的组都暴露在由 装有二向色扩散器的聚光灯 石英灯 10只小鼠的哨兵组在0、6、8、9、10、11、12、13、14、15、16、17、18、19、 开始UV处理后10、12、14、16、18和20周， 背部皮肤组织学检查。 它们背部的皮肤 组织学检查。 背部皮肤的条纹是数字成像的， 沿着纵向方向的沿着帧， 宽度为150微米，高度足以包括 皮肤 在每帧的基础上测量多个纹理特征; 每个特征测量应根据 与在正常皮肤中进行的平均测量的偏差，例如，“百分比 200帧与相同帧的平均值偏离超过4个SD单位 在正常皮肤中进行测量”。 纹理特征包括马尔可夫 转移概率值和其他函数， 与正常组织平均值的偏差。 有丝分裂象数目 应报告沿食管基底层沿着测量的每mm。

INHIBITION OF UV-INDUCED SKIN CARCINOGENESIS IN SKH HAIRLESS MICE; SCREENING AND RANKING OF CHEMOPREVENTIVE. This project measures the inhibition of UV-induced skin carcinogenesis in SKH hairless mice to screen and rank chemopreventive agents. The studies utilize compute- assisted quantitative image analysis (CIA) to measure a broad range of specialized chromatin texture features. SKH hairless mice, 6-8 weeks of age and weighing 18-22 grams, are being used. The control group is being exposed to a natural 12 hr/dark, 12/hr light cycle of window filtered sunlight. All remaining groups are being exposed to light emitted by spotlights equipped with dichroic diffusers incorporating tungsten halogen quartz lamps. Sentinel groups of 10 mice are being sacrificed at 0, 6, 8, 10, 12, 14, 16, 18 and 20 weeks after start of UV treatment and strips of their dorsal skin examined histologically. Strips of their dorsal skin examined histologically. Strips of dorsal skin are digitally imaged in contiguous frames along the longitudinal direction that are approximately 150 microns in width and high enough to include the full thickness of the skin. Multiple texture features are being measured on a per frame basis; each feature measurement shall be reported in terms of the degree of deviation from the mean measurement made in normal skin, e.g., "percent of 200 frames deviating more than 4 SD units from the mean of the same measurement made in normal skin". Texture features include Markovian transition probability values and other functions which show the greatest deviation from the mean in normal tissues. the number of mitotic figures per mm measured along the basal layer of the esophagus shall be reported.