ANIMAL FACILITIES--PERFORMANCE OF EXPERIMENTS AND TESTS

动物设施——实验和测试的进行

• 批准号: 3725536
• 负责人: PHILLIP D DR MARKHAM
• 金额: $ 51.33万
• 依托单位: ADVANCED BIOSCIENCE LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3725536
• 关键词: Primates; animal care; animal colony; enzyme linked immunosorbent assay; goats; guinea pigs; immunological substance; laboratory mouse; laboratory rabbit; laboratory rat; tissue /cell culture; tissue resource /registry; virus classification; western blottings

The objectives of this project are several-fold. First, to provide an animal facility with BL3 capability, in order to maintain a variety of laboratory animals, including mice, rats, guinea pigs, rabbits, goats, and non-human primates (principally rhesus and pigtail macaques). Second, to provide housing, veterinary care, routine health surveillance and standard technical and veterinary assistance for performance of routine procedures necessary for animal experimentation. Third, to provide veterinary assistance for performance of surgical procedures and postmortem examinations, and clinical laboratory support for analysis of animal materials, including pathological examinations. Fourth, to provide personnel to carry out routine virological experiments including infecting animals with viruses, determining the viral infectivity and pathogenicity, characterizing immune responses, and conducting viral challenge experiments. Fifth, to provide BL2/BL3 laboratory facilities for carrying out cell biological and immunological experiments in support of the animal experimentation. Sixth, to carry out in vitro immunologic and cell biologic experiments, including virus isolation and titering, Western blotting, and ELISAs in support of the animal experiments. Seventh, to prepare, characterize, purify, and store monoclonal and polyclonal antibodies. Eighth, to conduct experiments of in vivo tumorigenicity, and passage and characterize any resultant tumors. Finally, to provide storage space at -7O degrees C and liquid nitrogen temperatures for tissues, body fluids, and cell cultures resulting from these experiments.

该项目的目标有几个方面。 第一，提供一个 动物设施与BL3能力，以保持各种 实验动物，包括小鼠、大鼠、豚鼠、兔子、山羊和 非人类灵长类动物（主要是恒河猴和猪尾猕猴）。 二是 提供住房、兽医护理、日常卫生监督和标准 为执行常规程序提供技术和兽医援助 动物实验所必需的。 第三，提供兽医 协助进行外科手术和尸检 检查和临床实验室支持动物分析 材料，包括病理检查。 第四，提供 进行常规病毒学实验，包括感染 携带病毒的动物，确定病毒的感染性和致病性， 表征免疫反应，并进行病毒攻击 实验 第五，提供BL2/BL3实验室设施， 支持动物的细胞生物学和免疫学实验 实验 第六，进行体外免疫和细胞 生物学实验，包括病毒分离和滴定，Western 印迹和ELISA以支持动物实验。 七是 制备、表征、纯化和储存单克隆和多克隆 抗体的第八，进行体内致瘤性实验， 并传代和表征任何产生的肿瘤。最后，提供 储存空间在-70摄氏度和液氮温度， 组织、体液和细胞培养物。