CORE--PATHOLOGY PROGRAM

病理学核心在补助金中发挥支持作用， 解剖学和血液病理学数据。 该小组有四项研究活动 包括：1）移植物抗宿主病（GVHD），2）间质性肺炎， 3)静脉闭塞性疾病;和4）病毒诊断组织学。 这些 活动涉及发病机制中的重要问题， 疾病的诊断和治疗方法 骨髓移植 活动1测试干细胞假说， GVHD靶向作为一种获得对GVHD发病机制的新见解的方式， 效应器水平的GVHD。 其目的是提供更好的预测 重视GVHD的特异性和敏感性试验， 发生在同基因和自体的假GVHD之间的区别 真正的GVHD患者 这包括预测早期GVHD的方法 在第21天之前 活动2解决了 非感染性肺炎组，进行支气管肺泡灌洗 检测这些条件的方法。 它预测了不同的发病机制 弥漫性肺泡损伤和间质性肺炎， 不同的疗法。 活动3提出了一种新的方法来预测 免疫组织化学研究显示肝静脉闭塞性疾病 有或无既往肝脏移植患者的肝活检 炎症 活动4涉及通过1）改进病毒诊断 聚合酶链反应检测小儿早期巨细胞病毒肺炎 支气管肺泡灌洗标本，2）病毒潜伏期的研究 检测早期抗原的基因，作为激活潜伏的 疾病，3）开发新的单链探针，以改善 病毒诊断，4）开发多种原位杂交筛查 使用pcr检测4种病毒（巨细胞病毒、HSV-1、HSV-2和VZV） 探针，和5）改进的Y染色体原位杂交检测， 使用Y和X染色体探针。

The Pathology Core plays a supportive role in the grant by providing anatomic and hematopathology data. The group has four research activities involving: 1) graft-versus-host disease (GVHD), 2) interstitial pneumonia, 3) veno-occlusive disease, and 4) viral diagnostic histology. These activities deal with important problems in the pathogenesis of, and methodology for improving the diagnosis and treatment of the diseases of bone marrow transplantation. Activity 1 tests the stem cell hypothesis of GVHD targeting as a way of gaining new insights into the pathogenesis of GVHD at the effector level. Its aims are to provide better predictive value specificity and sensitivity tests for GVHD and improve the distinction between pseudo-GVHD occurring in syngeneic and autologous patients and true GVHD. This includes a method of predicting early GVHD before day 21. Activity 2 addresses a new distinction within the noninfectious pneumonia group and deals with bronchoalveolar lavage detection methods for these conditions. It predicts different pathogenesis for diffuse alveolar damage and interstitial pneumonia and implies different therapies. Activity 3 proposes a new way to predict the risk of veno-occlusive disease of the liver based on immunohistological study of liver biopsies in transplant patients with and without prior liver inflammation. Activity 4 deals with improved viral diagnosis by 1) polymerase chain reaction detection of early cytomegalovirus pneumonia in bronchoalveolar lavage specimens, 2) the study of viral latency by detecting the genes for early antigens as signals for activation of latent disease, 3) the development of new single-stranded probes for improved viral diagnosis, 4) development of multiple in situ hybridization screening panel for four viruses (cytomegalovirus, HSV-1, HSV-2 and VZV) using pcr probes, and 5) improved Y chromosome in situ hybridization detection by the use of combined Y and X chromosome probes.