CORE--PATHOLOGY PROGRAM

核心--病理学课程

• 批准号: 3771409
• 负责人: GEORGE E SALE
• 金额: --
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3771409
• 关键词: Adenoviridae; Betaherpesvirinae; Herpes simplex disease; autologous transplantation; biomedical facility; biopsy; bone marrow exam; bone marrow transplantation; communicable disease diagnosis; diagnosis service; dogs; graft versus host disease; herpes simplex virus 1; herpes simplex virus 2; histopathology; homologous transplantation; human subject; immunoelectron microscopy; immunofluorescence technique; immunopathology diagnosis; in situ hybridization; leukemia; nucleic acid probes; polymerase chain reaction; rapid diagnosis; viral pneumonia; virus classification

The Pathology Core plays a supportive role in the grant by providing anatomic and hematopathology data. The group has four research activities involving: 1) graft-versus-host disease (GVHD), 2) interstitial pneumonia, 3) veno-occlusive disease, and 4) viral diagnostic histology. These activities deal with important problems in the pathogenesis of, and methodology for improving the diagnosis and treatment of the diseases of bone marrow transplantation. Activity 1 tests the stem cell hypothesis of GVHD targeting as a way of gaining new insights into the pathogenesis of GVHD at the effector level. Its aims are to provide better predictive value specificity and sensitivity tests for GVHD and improve the distinction between pseudo-GVHD occurring in syngeneic and autologous patients and true GVHD. This includes a method of predicting early GVHD before day 21. Activity 2 addresses a new distinction within the noninfectious pneumonia group and deals with bronchoalveolar lavage detection methods for these conditions. It predicts different pathogenesis for diffuse alveolar damage and interstitial pneumonia and implies different therapies. Activity 3 proposes a new way to predict the risk of veno-occlusive disease of the liver based on immunohistological study of liver biopsies in transplant patients with and without prior liver inflammation. Activity 4 deals with improved viral diagnosis by 1) polymerase chain reaction detection of early cytomegalovirus pneumonia in bronchoalveolar lavage specimens, 2) the study of viral latency by detecting the genes for early antigens as signals for activation of latent disease, 3) the development of new single-stranded probes for improved viral diagnosis, 4) development of multiple in situ hybridization screening panel for four viruses (cytomegalovirus, HSV-1, HSV-2 and VZV) using pcr probes, and 5) improved Y chromosome in situ hybridization detection by the use of combined Y and X chromosome probes.

病理学核心在资助中发挥了支持作用，它提供了 解剖学和血液病理学数据。该小组有四项研究活动 涉及：1)移植物抗宿主病(GVHD)，2)间质性肺炎， 3)静脉闭塞疾病；4)病毒诊断组织学。这些 活动涉及致病机制中的重要问题，以及 提高脑血管疾病诊断和治疗水平的方法 骨髓移植。活动1检验了干细胞假说 GVHD靶向作为一种新的方式获得对GVHD发病机制的新见解 效应器水平的GVHD。其目的是提供更好的预测性 GVHD的价值特异性和敏感性测试，并改善 同基因和自体的假性移植物抗宿主病的鉴别 病人和真正的移植物抗宿主病。这包括一种预测早期GVHD的方法 在第21天之前。活动2解决了 非感染性肺炎组，处理支气管肺泡灌洗 针对这些情况的检测方法。它预测了不同的发病机制 弥漫性肺泡损伤和间质性肺炎 不同的疗法。活动3提出了一种预测风险的新方法 肝静脉闭塞症的免疫组织学研究 有肝和无肝移植患者的肝活检 发炎。活动4涉及改进的病毒诊断1) 聚合酶链式反应检测儿童早期巨细胞病毒肺炎 支气管肺泡灌洗标本，2)病毒潜伏期的研究 检测早期抗原基因作为潜伏激活信号 疾病，3)新的单链探针的开发 病毒诊断，4)多重原位杂交筛选技术的发展 四种病毒(巨细胞病毒、HSV-1、HSV-2和VZV)的聚合酶链式反应 5)改进了Y染色体原位杂交检测方法 联合使用Y和X染色体探针。