RETROVIRUSES--REPLICATION, GENETICS, AND EVOLUTION

逆转录病毒——复制、遗传学和进化

• 批准号: 3729247
• 负责人: HOWARD M TEMIN
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3729247
• 关键词: RNA directed DNA polymerase; Retroviridae; Retroviridae disease; chickens; gene mutation; genetic recombination; human immunodeficiency virus; molecular cloning; mutant; natural selections; neoplastic transformation; nucleic acid repetitive sequence; nucleic acid sequence; polymerase chain reaction; provirus; recombinant DNA; site directed mutagenesis; tissue /cell culture; transfection; viral carcinogenesis; virion; virus envelope; virus genetics; virus replication

Retrovirus variation is an important component of retrovirus carcinogenesis and of the AIDS epidemic. Dr. Temin's group is using specially designed retrovirus vectors and helper cells to study the genetics of retroviruses and the rates an mechanisms of retrovirus variation. They have shown that base pair substitutions, frame-shifts, simple deletions, complex rearrangements, homologous recombination, and nonhomologous recombination occur at rates of 1 x 10-5, 7 x 10-7, 2 x 10- 6, 1 x 10-6, 4 x 10-5, and 4 x 10-8 per bp per cycle, respectively, for a simple C-type retrovirus. They propose further analysis of retrovirus mutation with different subfamilies of simple and complex retroviruses to determine whether these rates are the same or different and to determine what factors affect these rates. They will also further characterize retrovirus RNA dimerization, encapsidation, strand switching in reverse transcription, and recombination with simple retroviruses, and they will characterize recombination in complex retroviruses. These studies will give a more complete understanding of retrovirus genetics and the processes involved in retrovirus evolution.

逆转录病毒变异是逆转录病毒的重要组成部分 癌症的发生和艾滋病的流行。特明博士的团队正在使用 专门设计的逆转录病毒载体和辅助细胞来研究 逆转录病毒的遗传学及逆转录病毒的发病机制 变种。他们已经证明了碱基对替换，框架移动， 简单的缺失、复杂的重排、同源重组和 非同源重组以1×10-5、7×10-7、2×10-5的速率发生 每个BP每周期分别为6、1 x 10-6、4 x 10-5和4 x 10-8 一种简单的C型逆转录病毒。他们建议对逆转录病毒进行进一步的分析 简单和复杂逆转录病毒不同亚家族的突变 以确定这些比率是否相同或不同，并 确定哪些因素会影响这些比率。他们还将进一步 逆转录病毒RNA二聚化、囊化、链交换的特征 逆转录，并与简单的逆转录病毒重组，以及 他们将描述复杂逆转录病毒中的重组。这些 研究将使我们对逆转录病毒的遗传学有更全面的了解 以及逆转录病毒进化所涉及的过程。