RETROVIRUSES--REPLICATION, GENETICS, AND EVOLUTION

逆转录病毒——复制、遗传学和进化

• 批准号: 6236463
• 负责人: HOWARD M TEMIN
• 金额: $ 25.66万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6236463
• 关键词: RNA directed DNA polymerase; Retroviridae; Retroviridae disease; bovine leukemia virus; chickens; gene mutation; genetic recombination; human immunodeficiency virus; molecular cloning; mutant; natural selections; neoplastic transformation; nucleic acid repetitive sequence; nucleic acid sequence; polymerase chain reaction; provirus; recombinant DNA; site directed mutagenesis; tissue /cell culture; transfection; viral carcinogenesis; virion; virus envelope; virus genetics; virus replication

Retrovirus variation is an important component of retrovirus carcinogenesis and of the AIDS epidemic. Dr. Temin's group is using specially designed retrovirus vectors and helper cells to study the genetics of retroviruses and the rates an mechanisms of retrovirus variation. They have shown that base pair substitutions, frame-shifts, simple deletions, complex rearrangements, homologous recombination, and nonhomologous recombination occur at rates of 1 x 10-5, 7 x 10-7, 2 x 10- 6, 1 x 10-6, 4 x 10-5, and 4 x 10-8 per bp per cycle, respectively, for a simple C-type retrovirus. They propose further analysis of retrovirus mutation with different subfamilies of simple and complex retroviruses to determine whether these rates are the same or different and to determine what factors affect these rates. They will also further characterize retrovirus RNA dimerization, encapsidation, strand switching in reverse transcription, and recombination with simple retroviruses, and they will characterize recombination in complex retroviruses. These studies will give a more complete understanding of retrovirus genetics and the processes involved in retrovirus evolution.

逆转录病毒变异是逆转录病毒的重要组成部分 癌症发生和艾滋病流行。 Temin 博士的小组正在使用 专门设计的逆转录病毒载体和辅助细胞来研究 逆转录病毒的遗传学以及逆转录病毒的发生率和机制 变化。 他们已经证明碱基对替换、移码、 简单的删除、复杂的重排、同源重组、 非同源重组的发生率为 1 x 10-5、7 x 10-7、2 x 10- 每个周期每个 bp 分别为 6、1 x 10-6、4 x 10-5 和 4 x 10-8 一种简单的C型逆转录病毒。 他们建议进一步分析逆转录病毒 简单和复杂逆转录病毒不同亚科的突变 确定这些费率是相同还是不同，并 确定哪些因素影响这些比率。 他们还将进一步 表征逆转录病毒 RNA 二聚化、衣壳化、链转换 逆转录，以及与简单逆转录病毒的重组，以及 他们将表征复杂逆转录病毒中的重组。 这些 研究将更全面地了解逆转录病毒遗传学 以及逆转录病毒进化所涉及的过程。