RETROVIRUSES--REPLICATION, GENETICS, AND EVOLUTION

逆转录病毒——复制、遗传学和进化

• 批准号: 3749335
• 负责人: HOWARD M TEMIN
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3749335
• 关键词: RNA directed DNA polymerase; Retroviridae; Retroviridae disease; chickens; gene mutation; genetic recombination; human immunodeficiency virus; molecular cloning; mutant; natural selections; neoplastic transformation; nucleic acid repetitive sequence; nucleic acid sequence; polymerase chain reaction; provirus; recombinant DNA; site directed mutagenesis; tissue /cell culture; transfection; viral carcinogenesis; virion; virus envelope; virus genetics; virus replication

Retrovirus variation is an important component of retrovirus carcinogenesis and of the AIDS epidemic. Dr. Temin's group is using specially designed retrovirus vectors and helper cells to study the genetics of retroviruses and the rates an mechanisms of retrovirus variation. They have shown that base pair substitutions, frame-shifts, simple deletions, complex rearrangements, homologous recombination, and nonhomologous recombination occur at rates of 1 x 10-5, 7 x 10-7, 2 x 10- 6, 1 x 10-6, 4 x 10-5, and 4 x 10-8 per bp per cycle, respectively, for a simple C-type retrovirus. They propose further analysis of retrovirus mutation with different subfamilies of simple and complex retroviruses to determine whether these rates are the same or different and to determine what factors affect these rates. They will also further characterize retrovirus RNA dimerization, encapsidation, strand switching in reverse transcription, and recombination with simple retroviruses, and they will characterize recombination in complex retroviruses. These studies will give a more complete understanding of retrovirus genetics and the processes involved in retrovirus evolution.

逆转录病毒变异是逆转录病毒的重要组成部分