CORE--VIROLOGY CORE LABORATORY

核心--病毒学核心实验室

• 批准号: 3727425
• 负责人: MOSTAFA NOKTA
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3727425
• 关键词: Adenoviridae; Epstein Barr virus; HIV envelope protein gp120; HIV infections; acyclovir; antiAIDS agent; biomedical facility; blood tests; cooperative study; cytomegalovirus; drug tolerance; ganciclovir; herpes simplex virus 1; herpes simplex virus 2; human immunodeficiency virus; human subject; microorganism disease chemotherapy; opportunistic infections; urinalysis; virus antigen; virus diseases

The core virology laboratory will be responsible for performing protocol mandated virology testing. This will serve the ACTU in this institution as well as other ACTG units without virology core laboratories. HIV, CMV, EBV, VZV, and HSV serologic studies will be performed at baseline and thereafter as mandated by the clinical protocol. Primarily, initial baseline virology determinations will be done before the initiation of therapy, and at frequent intervals thereafter, these will include virus isolations and HIV serum P-24 Ag determinations. HIV isolations will be from peripheral blood, unless protocol mandates otherwise. HIV plasma viremia may be determined, when indicated, as a potential marker of antiretroviral therapeutic efficacy. The virology core will also isolate and quantify other opportunistic viruses that commonly infect HIV patients, particularly cytomegalovirus (CMV), herpes simplex virus (HSV), and adenovirus. Quantitative CMV urine cultures are available for use as a marker of anticytomegaloviral therapeutic efficacy. All isolates recovered from patients on specific antiviral therapy will be stored for use, as needed, for antiviral resistance testing. Rapid CMV culture recovery may be facilitated using the shell vial centrifugation method. Sensitivity testing of patient derived viral isolates to antiviral compounds (e.g. ganciclovir-resistant CMV and acyclovir-resistant HSV) will be done as needed. Measurement of other surrogate markers of HIV markers as gp120, nef protein, neopterin, B2 microglobulin, and C1q and other markers as indicated by study protocols. Moreover, the laboratory will continue to be involved in the development and evaluation of novel virology diagnostic tools as they become available and in the modification of existent methodologies to increase their sensitivities and specificities. The laboratory will also participate in the quality assurance program established by the ACTG in order to standardize the results obtained.

核心病毒学实验室将负责执行方案