3D conducting polymer devices for hosting and monitoring human gut models with integrated fluidics

3D 导电聚合物设备，用于通过集成流体技术托管和监测人体肠道模型

• 批准号: EP/X02833X/1
• 负责人: Roisin Owens
• 金额: $ 16.47万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX02833X%2F1
• 关键词: 3D; conducting; polymer; devices; hosting

Organ-on-chip (OoC) technologies have the potential to revolutionise the drug testing and toxicology industries, not just by stream-lining early stages of pre-clinical testing and reducing costly (and sometimes irrelevant) experimentation on animals, but also by offering real hope for personalised medicine. Despite this promise, current OoC models fail to fully capture human physiological complexity and suffer from lack of integration of monitoring technology into the platforms. Recent progress in the IMBIBE ERC consolidator project has shown that 3D porous electrodes can be used to host and monitor tissues representative of the human gut. These devices are capable of hosting multi-cellular models of the human gut, including microbiota, now shown to be an integral part of human biology, particularly in the gut. Cells, human and microbe, are hosted within a sponge-like electrode that mimics the in vivo environment. As they grow, cells are dynamically monitored by the electrode, whose electrical properties change due to cell attachment and tissue differentiation. We have established a collaboration with CN Bio Innovations, a leading bioengineering company specialising in single and multi-organ OoC platforms and innovative lab technologies, to adapt our device to their state of the art fluidic platform. The PhysioMimix Organ-on-Chip (PMX-OOC) developed by CN Bio is a flexible and simple to use OoC system which uses a range of different multi-well consumable plates to develop single and multi-organ in vitro models. The result will be a sophisticated bioelectronic device capable of hosting and monitoring a complex human model of the gut (under flow conditions). We anticipate that the result will be a predictive, accurate and high throughput platform which will be very attractive to the pharmaceutical industry for cost-effective and efficient drug discovery, toxicity testing, post market surveillance and development of live biotherapeutics.

器官片（OOC）技术有潜力彻底改变药物测试和毒理学行业，这不仅是临床前测试的早期阶段，并减少了对动物的代价高昂（有时是无关）的实验，而且还通过为个性化医学提供了真正的希望。尽管有这样的承诺，目前的OOC模型仍未完全捕获人类的生理复杂性，并且由于缺乏监测技术的整合到平台中。 Imbibe ERC合并项目的最新进展表明，3D多孔电极可用于托管和监测代表人类肠道的组织。这些设备能够托管人类肠道的多细胞模型，包括微生物群，现在被证明是人类生物学不可或缺的一部分，尤其是在肠道中。人类和微生物的细胞托管在模拟体内环境的海绵样电极中。随着它们的生长，电极会动态监测细胞，其电气特性由于细胞附着和组织分化而变化。我们已经与CN Bio Innovations建立了合作，该公司是一家领先的生物工程公司，专门研究单一和多器官OOC平台和创新的实验室技术，以使我们的设备适应其最先进的流体平台。 CN Bio开发的Physiomimix On-Chip（PMX-OOC）是一种灵活且易于使用的OOC系统，它使用一系列不同的多孔消耗板来开发单个和多器官的体外模型。结果将是一个复杂的生物电子设备，能够托管和监测肠道的复杂人类模型（在流动条件下）。我们预计结果将是一个预测，准确且高吞吐量的平台，对制药行业将非常有吸引力的药物发现，毒性测试，市场监测和实时生物治疗学的开发。