SCOR IN SUDDEN CARDIAC DEATH

我们建议成立一个专门的猝死研究中心 犹他大学调查异常之间的关系 复极和心律失常性死亡。调查小组是 由17名调查员和8名顾问组成。四个研究项目 并提出了四个核心支持实验室。第一项研究 人体心肌细胞复极的离子机制 在MC Sanguinetti的指导下，心肌细胞以K+和其他 使电流重新极化。电压钳位技术应用于隔离 以确定存在哪些电流，以充分 描述它们的特征，并确定它们如何影响正常和异常 复极化过程。在项目2中，分子和生理 长QT综合征的机制，MT Kating和他的同事研究 此病的分子遗传学和心脏电生理 人类和人类心肌中的遗传性心律失常。JW Mason 同事们计划对发生的复极变化进行研究 紧接在第三个项目中因缺血猝死之前， 与室性心律失常和突发事件相关的复极改变 死亡。在项目4中，心电图室测量 复极化--非侵入性区域测量技术 心脏复极和复极差异是由 LS Green，RL Lux和他的同事对男性进行诊断和预测。 由梅森领导的行政核心将向所有人提供帮助 预算管理和内部沟通方面的调查人员。核心2，即 WH Barry和GA Peeters领导的细胞处理实验室，Will 为项目1和项目2准备分离的人心室肌细胞， 为项目1维护和培养青蛙卵母细胞，并转化和 LQT患者外周血淋巴细胞DNA的维持 项目2.核心3复极化信号处理的分析 由Lux领导的实验室将提供心电信号 项目2、3和4的处理和口译能力 文在寅领导第四个核心实验室，临床数据管理和 生物统计学，它将协助项目2、3和4的调查人员 临床数据的收集、统计分析和解释 从病人那里获得的。 这项SCOR提案尖锐地聚焦于问题的一个方面 突然死亡的恐惧。它结合了基础科学和整体器官生理学。 和复杂的临床医学。它可能会让人们深入了解 猝死的病理生理学和导致治疗的改进 为未来的基本发现建立一个框架。

We propose a Specialized Center of Research in Sudden Death at the University of Utah to investigate the relationship between abnormalities of repolarization and arrhythmic death. The investigative group is composed of 17 investigators and 8 consultants. Four Research Projects and four Core support laboratories are proposed. The first research project, lonic Mechanisms of Repolarization in Human Ventricular Myocytes, directed by MC Sanguinetti, centers on K+ and other repolarizing currents. Voltage clamp techniques are applied to isolated human myocytes to determine which currents are present, to fully characterize them and to determine how they influence normal and abnormal repolarization processes. In Project 2, Molecular and Physiologic Mechanisms of the Long QT Syndrome, MT Keating and colleagues investigate the molecular genetics and the cardiac electrophysiology of this inherited form of arrhythmia in man and in human myocardium. JW Mason and coworkers plan studies of repolarization changes that occur immediately preceding sudden death due to ischemia in the third project, Repolarization Changes Associated with Ventricular Arrhythmias and Sudden Death. In Project 4, Electrocardiographic Measures of Ventricular Repolarization, techniques for non-invasive measurement of regional cardiac repolarization and disparity of repolarization are developed by LS Green, RL Lux and colleagues for diagnosis and prognostication in man. The Administrative Core, led by Mason, will provide assistance to all investigators in budget management and intercommunication. Core 2, the Cell Processing Laboratory directed by WH Barry and GA Peeters, will prepare isolated human ventricular myocytes for Projects 1 and 2, maintain and culture frog oocytes for Project 1, and transform and maintain peripheral blood lymphocytes from patients with LQT for DNA analysis in Project 2. Core 3, the Repolarization Signal Processing Laboratory led by Lux, will provide electrocardiographic signal processing and interpretation capabilities to Projects 2, 3 and 4. TE Moon directs the fourth Core laboratory, Clinical Data Management and Biostatistics, which will assist Project 2, 3 and 4 investigators in collection, statistical analysis and interpretation of clinical data obtained from patients. This SCOR proposal is sharply focussed on a single facet of the problem of sudden death. It combines basic science with whole-organ physiology and complex clinical medicine. It is likely to produce insight into the pathophysiology of sudden death and lead to improvements in therapy while establishing a framework for future fundamental discoveries.