SCOR IN SUDDEN CARDIAC DEATH

我们建议在猝死研究的专业中心， 犹他州大学研究异常之间的关系 复极化和死亡的可能性 调查小组是 调查员17名，顾问8名。 四个研究项目 四个核心支撑实验室。 第一研究 人体心室肌复极离子机制 由MC Sanguinetti指导的肌细胞以K+和其他 复极化电流 电压钳位技术应用于隔离 人类心肌细胞，以确定哪些电流存在，以充分 并确定它们如何影响正常和异常 复极化过程 在项目2中，分子和生理 MT Keating及其同事研究了长QT综合征的机制， 分子遗传学和心脏电生理学 人类和人类心肌遗传性心律失常。 JW梅森 和同事们计划研究发生的复极变化 在第三个项目中由于缺血而猝死之前， 室性心律失常和突发性心律失常的复极改变 死亡 在项目4中，心室的心电图测量 复极，用于非侵入性测量区域 心脏复极和复极不一致是由 LS绿色，RL勒克斯和同事的诊断和人体内。 梅森领导的行政核心将为所有人提供援助， 预算管理和内部沟通方面的调查员。 核心2， 细胞处理实验室由WH巴里和GA彼得斯指导，将 为项目1和2制备分离的人心室肌细胞， 项目1蛙卵母细胞的维持和培养， 维持LQT患者外周血淋巴细胞DNA 项目分析2。 核心3，复极化信号处理 由Lux领导的实验室将提供心电图信号 项目2、3和4的处理和解释能力。 TE Moon领导第四个核心实验室，临床数据管理和 生物统计学，将协助项目2、3和4的研究人员 临床数据的收集、统计分析和解释 从患者身上获得。 SCOR的建议集中在问题的一个方面 突然死亡。 它结合了基础科学和全器官生理学 复杂的临床医学。 它很可能会产生对 猝死的病理生理学，并导致治疗的改善， 为未来的基本发现建立一个框架。

We propose a Specialized Center of Research in Sudden Death at the University of Utah to investigate the relationship between abnormalities of repolarization and arrhythmic death. The investigative group is composed of 17 investigators and 8 consultants. Four Research Projects and four Core support laboratories are proposed. The first research project, lonic Mechanisms of Repolarization in Human Ventricular Myocytes, directed by MC Sanguinetti, centers on K+ and other repolarizing currents. Voltage clamp techniques are applied to isolated human myocytes to determine which currents are present, to fully characterize them and to determine how they influence normal and abnormal repolarization processes. In Project 2, Molecular and Physiologic Mechanisms of the Long QT Syndrome, MT Keating and colleagues investigate the molecular genetics and the cardiac electrophysiology of this inherited form of arrhythmia in man and in human myocardium. JW Mason and coworkers plan studies of repolarization changes that occur immediately preceding sudden death due to ischemia in the third project, Repolarization Changes Associated with Ventricular Arrhythmias and Sudden Death. In Project 4, Electrocardiographic Measures of Ventricular Repolarization, techniques for non-invasive measurement of regional cardiac repolarization and disparity of repolarization are developed by LS Green, RL Lux and colleagues for diagnosis and prognostication in man. The Administrative Core, led by Mason, will provide assistance to all investigators in budget management and intercommunication. Core 2, the Cell Processing Laboratory directed by WH Barry and GA Peeters, will prepare isolated human ventricular myocytes for Projects 1 and 2, maintain and culture frog oocytes for Project 1, and transform and maintain peripheral blood lymphocytes from patients with LQT for DNA analysis in Project 2. Core 3, the Repolarization Signal Processing Laboratory led by Lux, will provide electrocardiographic signal processing and interpretation capabilities to Projects 2, 3 and 4. TE Moon directs the fourth Core laboratory, Clinical Data Management and Biostatistics, which will assist Project 2, 3 and 4 investigators in collection, statistical analysis and interpretation of clinical data obtained from patients. This SCOR proposal is sharply focussed on a single facet of the problem of sudden death. It combines basic science with whole-organ physiology and complex clinical medicine. It is likely to produce insight into the pathophysiology of sudden death and lead to improvements in therapy while establishing a framework for future fundamental discoveries.