LIVER BIOPSY ASSOCIATED WITH CHOLECYSTECTOMY IN A SINGLE PATIENT

单个患者与胆囊切除术相关的肝活检

• 批准号: 3741298
• 负责人: STEPHEN YOUNG
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3741298
• 关键词: abetalipoproteinemias; apolipoproteins; biopsy; blood lipoprotein biosynthesis; cholecystectomy; familial hypolipoproteinemia; gene mutation; human subject; liver cells; liver metabolism; messenger RNA; radiotracer; tissue /cell culture

Hepatocytes will be obtained from a liver biopsy specimen at the time of an elective cholecystectomy to analyze the relative levels of mutant and normal apolipoprotein B mRNA levels in the hepatocytes of a single individual who is heterozygous for familial hypobetalipoproteinemia. The relative rates of synthesis of the truncated (mutant) apo-B protein and the full-length apo-B protein will also be determined by 35S metabolic labeling studies in cultured hepatocytes.

将在以下时间从肝脏活检样本中获取肝细胞 一个选择性胆囊切除术，以分析突变的相对水平， 正常载脂蛋白B mRNA水平在肝细胞中的单个 家族性低β脂蛋白血症杂合子个体。 的 截短（突变）apo-B蛋白的相对合成速率， 全长apo-B蛋白也将通过35 S代谢测定 在培养的肝细胞中的标记研究。