Emerging nanoscopy for single entity characterisation

在纳米级水平上解释现象的能力导致了对单个实体的结构和机制的前所未有的和精细的理解。这在生物医学、生物物理学和生物材料纳米科学领域带来了一个新时代，从而修正了我们以前对细胞结构和纳米电子学的概念。这些技术具有巨大的潜力，不仅可以改变我们知识的进步，还可以改变诊断/预后方法的发展。然而，我们目前缺乏在这个具有挑战性的维度上进行相关成像的能力，同时将纳米级的机械，物理和电学参数与宏观现象直接联系起来。因此，探索创新的测量和成像方法是及时和重要的，它可以克服传统路线的局限性，并成为第二次相关纳米革命的使能技术。拟议的“新兴纳米显微镜单一实体表征（ENSIGN）”项目就是这样一种新颖的方法，旨在开发一种转型的，综合的方法，用于单一实体成像和表征。ENSIGN将开发并联合收割机将高速力、电和微波纳米显微镜与光学和电子纳米显微镜结合起来，提供定量、同时多参数测量、高速且具有成本效益的超越下一代最先进的能力单一实体成像、电化学、机械生物学和生物力学。开发的纳米显微镜将具有前所未有的高分辨率，多模式和多维同时成像能力，并且是定量，快速和非侵入性的。所获得的先进技术将成为细胞生物学、纳米材料和下一代电池进步的基石，从而保持欧洲在这些研究领域潜在的重大科学和技术突破方面的世界领先地位。

The capability of interpreting phenomena at the nanoscale level has led to an unprecedented and refined understanding of structures and mechanisms of single entities. This has brought a new era across the fields of biomedicine, biophysics and biomaterial nanoscience, and thus revise our previous concepts on cellular structures and nanoscale electronics. These technologies bear an enormous potential to transform not only the advancement of our knowledge, but also the development of diagnostic/prognostic approaches. However, we currently lack the ability to conduct correlative imaging at this challenging dimension while directly linking the nanoscale mechanical, physical and electrical parameters with macroscopic phenomena. Therefore, it is timely and important to explore innovative measurement and imaging methods, which could overcome the limitations of conventional routes and become enabling technologies for the second correlative nanoscopy revolution. The proposed 'Emerging nanoscopy for single entity characterisation (ENSIGN)' project is such a novel approach, which seeks to develop a transformational, integrated approach for single entity imaging and characterisation. ENSIGN will develop and combine high speed force, electrical, and microwave nanoscopy with optical and electron nanoscopy, to provide a quantitative, simultaneous multiparameter measurement, high speed and cost-effective beyond state-of-the-art capabilities for next generation single entity imaging, electrochemistry, mechanobiology and biomechanics. The developed nanoscopy will have unprecedented high resolution, multi-modal and multi-dimensional simultaneous imaging capabilities and be quantitative, fast and non-invasive. The obtained advanced technique will form a cornerstone for the advancement of cell biology, nanomaterials, and next generation battery, and thus keep Europe's leading position in the world for potential major scientific and technological breakthroughs in these research areas.