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TROPICAL DISEASE RESEARCH UNIT

热带病研究室

基本信息

批准号：
2060209
负责人：
James Walter Kazura
金额：
$ 66.19万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-07-01 至 1995-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2060209
关键词：
filariasis host organism interaction immunity schistosomiasis tropical medicine

项目摘要

The overall objective of this renewal application for a Tropical Disease Research Unit is to examine specific molecular events critical to development and function of filarial parasites and Schistosoma mansoni and apply this knowledge and associated technologies to the design of anti- helminthic vaccines. The current application is a mutlidisciplinary collaborative program that integrates the scientific approaches nad technical tools of immunology, molecular biology, biochemistry, cell biology, and parasitology applied to study specific aspects of lymphatic filariasis and schistosomiasis mansoni. The specific aims of the four projects included are to: Project 1 . Define specific epitopes of a recombinant filarial antigen which induce protective immunity against B. malayi infection. 2. Elucidate the immune mechanisms by which protective filarial antigens induce resistance. Project 2 1. Define the genes in B. malayi and Ascaris which give rise to transcripts which are trans-spliced. 2. Define the sequence elements which direct initiation and termination of the short non-polyadenylated RNA from which the spliced leader exon is derived. Project 3 1. Define the biochemical structure and clone a 68K protective S. mansoni antigen. 2. Elucidate the immunologic basis of 68K vaccine-induced resistance and determine methods to enhance its efficacy. Project 4 1. Characterize the biochemical nature of the S. mansoni cercarial glycocalyx. 2. Study the mechanisms of glycocalyx release during transformation of cercariae to schistosomula. These projects are based on studies in experimental animals and sera from human volunteers and utilize in vitro manipulation of the causative organisms. Achievement of the goals of each is dependent on scientific and technical interactions between Projects 1-2, Projects 1-3, Projects 2-3, and Projects 3-4 and core facilities providing helminths, lymphocytes cloning expertise, and ultrastructure analyses.

热带病延续申请的总体目标研究单位是检查特定的分子事件，丝虫和曼氏血吸虫的发育和功能将这些知识和相关技术应用于反蠕虫疫苗当前的应用程序是一个多学科合作计划，将科学方法和免疫学、分子生物学、生物化学、细胞生物学生物学和寄生虫学应用于研究淋巴细胞的特定方面，丝虫病和曼氏血吸虫病四个具体目标包括的项目有：项目1 . 确定重组丝虫抗原的特异性表位其诱导针对B的保护性免疫。马来传染病。 2. 阐明免疫机制，丝虫抗原诱导抗性。计划2 1. 定义B中的基因。马来人和蛔虫，产生反式剪接的转录本。 2. 定义指导启动的序列元素，终止短的非多聚腺苷酸化的RNA，前导外显子是衍生的。项目3 1. 定义生化结构并克隆一个68 K 保护性S.曼氏抗原 2. 阐明68 K疫苗诱导的免疫学基础抗性和确定方法，以提高其效力。项目4 1. 描述了S.曼氏尾蚴糖萼 2. 研究糖萼释放的机制尾蚴向童虫的转化。这些项目是基于对实验动物和血清的研究，人类志愿者和利用体外操纵的致病有机体每个目标的实现都依赖于科学和项目1-2，项目1-3，项目2-3，和项目3-4以及提供蠕虫、淋巴细胞克隆技术和超微结构分析

项目成果

期刊论文数量（85）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Inhibition of surface membrane maturation in schistosomula of Schistosoma mansoni.

DOI：
10.1073/pnas.85.11.3825
发表时间：
1988-06
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
P. Wiest;A. Tartakoff;M. Aikawa;A. Mahmoud
通讯作者：
P. Wiest;A. Tartakoff;M. Aikawa;A. Mahmoud

Induction of murine T-helper-cell responses to the filarial nematode Brugia malayi.

诱导小鼠 T 辅助细胞对丝虫线虫马来丝虫的反应。