EVALUATION OF USEFULNESS OF PCR FOR DIAGNOSIS OF PNEUMOCYSTIS CARINII PNEUMONIA

PCR 诊断卡氏肺囊虫肺炎的有效性评估

• 批准号: 3752266
• 负责人: C P CARTWRIGHT
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752266
• 关键词: Pneumocystis carinii; Pneumocystis pneumonia; bacterial DNA; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; enzyme linked immunosorbent assay; polymerase chain reaction; rapid diagnosis; sputum

We developed a rapid, yet efficient procedure for extracting DNA from respiratory specimens, and a user friendly ELISA format PCR product detection assay. By combining these two technologies we were able to perform, in a diagnostic laboratory, a prospective evaluation of PCR as a method for diagnosing Pneumocystis carinii pneumonia (PCP). The results of this study indicate that the use of PCR could improve the efficiency of diagnosis of PCP, especially from non-invasive specimens (induced sputa). The improvement in diagnosis effected by PCR was particularly marked in patients that, a priori, might be expected to produce sputum specimens containing a low number of organisms (for example HIV patients receiving chemoprophylaxis, immunosuppressed non HIV patients).

我们开发了一种快速有效的方法从 呼吸道标本，以及用户友好的ELISA格式PCR产品 检测分析通过结合这两项技术，我们能够 在诊断实验室进行PCR的前瞻性评价 作为诊断卡氏肺孢子虫肺炎（PCP）的方法。的 这项研究的结果表明，使用PCR可以提高 PCP的诊断效率，特别是来自非侵入性标本的诊断效率 （induced arrhythmia）.通过PCR实现的诊断的改进是 特别是在患者中，先验地， 产生含有少量微生物的痰标本（用于 例如接受化学预防、免疫抑制、非 HIV患者）。