USE OF PCR RIBOTYPING FOR EPIDEMIOLOGICAL ANALYSIS OF CLOSTRIDIUM DIFFICILE

使用 PCR 核糖分型进行艰难梭菌流行病学分析

• 批准号: 5201190
• 负责人: C P CARTWRIGHT
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201190
• 关键词: Clostridium difficile; bacterial RNA; bacterial genetics; clinical chemistry; clostridial infection; diarrhea; epidemiology; microorganism classification; nosocomial infections; polymerase chain reaction; pulsed field gel electrophoresis; ribosomal RNA; western blottings

We demonstrated the feasibility, and epidemiologic validity of utilizing PCR ribotyping (amplication of the multiple-allelic intergenic spacer region of the rRNA gene cluster) as a technique for investigating the relatedness of isolates of C. difficile. We have, in collaboration with the Hospital Epidemiology Service, been involved in tracking potential outbreaks of C. difficile-associated diarrhea in the Clinical Center, and envisage that this close collaboration will continue. We are now examining, with the collaborative help of Dr. Matthew Samore, Deaconess Hospital, Boston, MA and Dr. Haru Kato, Gifu University School of Medicine, Gifu, Japan, the relative utility of a number of molecular epidemiologic approaches to typing C. difficile, including PCR ribotyping, arbitrary primed-PCR (AP-PCR), pulsed-field gel electrophoresis (PFGE) and immunoblotting. We envisage not only being able to make some reasonably definitive statements about the merits, or lack thereof, of these various techniques, but also to be in possession of a highly characterized set of C. difficile isolates. Such collections of isolates are invaluable when one seeks to evaluate new techniques for strain typing.

我们证明了利用这种方法的可行性和流行病学有效性， PCR核糖体分型（扩增多等位基因间隔区 rRNA基因簇的区域）作为研究 C.分离株的亲缘关系很难我们与 医院流行病学服务，一直参与跟踪潜在的 爆发C.临床中心的难治性腹泻，以及 预计这种密切合作将继续下去。我们现在 在女执事Matthew Samore博士的帮助下， 医院，波士顿，MA和博士Haru加藤，岐阜大学学院 日本岐阜医药的一些分子的相对效用 C型的流行病学方法。艰难梭菌，包括PCR核糖体分型， 随机引物PCR（AP-PCR）、脉冲场凝胶电泳（PFGE）和 免疫印迹。我们设想不仅能够合理地 关于这些不同的优点或缺乏优点的明确声明， 技术，但也要拥有一套高度特色的 C.艰难分离株。这种分离物的收集是无价的， 人们试图评估用于菌株分型的新技术。