EVALUATION OF USEFULNESS OF PCR FOR DIAGNOSIS OF PNEUMOCYSTIC INFECTION

PCR 诊断肺囊虫感染的有效性评估

• 批准号: 3774519
• 负责人: C P CARTWRIGHT
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774519
• 关键词: Pneumocystis pneumonia; diagnosis design /evaluation; diagnostic tests; enzyme linked immunosorbent assay; polymerase chain reaction; rapid diagnosis

Development of a PCR-based assay for diagnosis of Pneumocystis pneumonia. A simple, yet efficient, DNA extraction and amplification protocol has been developed, permitting this assay to be carried out in a single day on the Clinical Microbiology Laboratory. A prospective study is currently underway comparing PCR with FA for detection pf Pneumocystis in respiratory specimens, and preliminary data indicates that the PCR assay can improve the efficiency of diagnosis, specially for induced sputa. Of 160 specimens so far analyzed: 16 were PCR+/FA+; 6 were PCR+/FA- (all were sputum, subsequently confirmed), 0 were PCR-/FA+; 138 were PCR-/FA-. Work is currently underway to develop an ELISA-type assay for detection of the PCR product.

建立一种基于PCR的诊断肺孢子虫肺炎的方法。 一种简单而有效的DNA提取和扩增方法， 开发，允许在一天内进行该测定 临床微生物学实验室 一项前瞻性研究是 目前正在比较PCR和FA检测肺孢子虫 在呼吸道标本中，初步数据表明， 本试验可提高诊断效率，特别是对诱导型 你好 在迄今为止分析的160份标本中：16份为PCR+/FA+; 6份为 PCR+/FA-（均为痰液，随后确认），0例为PCR-/FA+; 138例 PCR-/FA-。 目前正在开发ELISA型测定法 用于检测PCR产物。