ANALYSIS OF CELLULAR FACTORS AFFECTING RETROVIRUS INFECTION AND GROWTH

ERF基因最初是根据其结合 ETS特异性DNA反应元件序列，后来被证明 作为ETS应答启动子的抑制子， 转染测定。 实验验证了电流变液是否能 抑制其启动子含有ETS的逆转录病毒的表达， 响应元件，并且在瞬时测定中，可以显示为 被ERF抑制。 已经表明，在共转染或共转染的细胞中， 用鼠白血病病毒（MuLV）衍生的病毒构建体感染， ERF，病毒RNA表达水平与对照组相比降低。 仅含有载体序列的对照细胞。 这些结果表明 ERF可能在体外作为病毒抑制剂。

The ERF gene was initially identified on the basis of its ability to bind to the ETS-specific DNA response element sequence, and was later shown to act as a suppressor of ETS-responsive promoters in transient transfection assays. Experiments were performed to test whether ERF can inhibit the expression of retroviruses whose promoters contain ETS- responsive elements and which, in transient assays, can be shown to be inhibited by ERF. It has been shown that in cells co-transfected or co- infected with a murine leukemia virus (MuLV)-derived viral construct and ERF, the level of viral RNA expression is reduced in comparison to control cells containing vector sequences only. These results suggest that ERF could potentially act as a virus inhibitor in vitro.