REGULATION OF EXPRESSION AND FUNCTION OF NEUROPEPTIDES DURING DEVELOPMENT

在过去的一年里，关于儿童发育表达的项目 非洲爪哇中枢神经系统的神经肽基因被终止。然而， LIM类家族一个成员的发育性表达 同源框基因在大鼠小脑中的原位研究 与V.Galo博士在LCMN合作进行的杂交。Northern印迹 分析表明，大鼠小脑中rLIM-1的表达处于高峰 出生后第8天水平与颗粒细胞高峰期一致 扩散。观察到rLIM-1显著下降，但内部 颗粒细胞表达低水平。在发育中的大鼠小脑中， 增殖和迁移前细胞高水平表达rLIM-1，但 在有丝分裂后细胞中下调，与 通过分子层的迁移。

In the past year, the project on the developmental expression of neuropeptide genes in the Xenopus CNS was terminated. However, the developmental expression of one member of the LIM class family of homeobox genes was characterized in rat cerebellum using in situ hybridization in collaboration with Dr. V. Gallo in LCMN. Northern blot analysis showed that rat rLIM-1 expression in cerebellum was at its peak level postnatal day 8, coincident with a peak period of granule cell proliferation. A dramatic decrease was observed in rLIM-1 but internal granule cells express low levels. In developing rat cerebellum, proliferative and premigratory cells express high levels of rLIM-1 but down regulates in post-mitotic cells, coincidental with onset of migration through the molecular layer.