Development of an innovative recombinant protein vaccine against Aeromonas veronii in European seabass using "omics" technologies (AeroVeroVacc)

使用“组学”技术开发针对欧洲鲈鱼维氏气单胞菌的创新重组蛋白疫苗 (AeroVeroVacc)

• 批准号: EP/Y026454/1
• 负责人: Kimberly Thompson
• 金额: $ 29.8万
• 依托单位: Moredun Research Institute
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY026454%2F1
• 关键词: Development; innovative; recombinant; protein; vaccine

Disease within aquaculture systems has led to significant economic and production losses and is one of the main barriers to expanding sustainable aquaculture. Vaccination is widely accepted as an effective strategy to combat pathogenic diseases in aquaculture and a valuable alternative to antibiotics. An emerging bacterial infection caused by Aeromonas veronii has been the cause of significant mortalities in seabass farms in the Aegean Sea. Screening for novel virulence factors in vivo may be one approach for identifying potential vaccine candidates for vaccine development against Aeromonas veronii. A novel immuno-screeningtechnique, in vivo induced antigen technology (IVIAT), a method that relies on antibodies adsorbed against in vitro cultures of the pathogen, is used to identify immunogenic proteins on bacteria exclusively expressed in vivo during infection. This study will utilise cutting-edge technologies to identify genes specifically expressed or upregulated in vivo within the host. In vivo induced (IVI) proteins common to the three clades of A. veronii affecting European seabass will be selected to design a universal recombinant protein vaccine for A. veronii infections affecting European seabass in the Mediterranean. The project's multidisciplinary nature is strong, involving a combination of microbiology, fish immunology, molecular biology, fish vaccinology, fish health, and aquaculture.This interdisciplinarity approach is necessary to fulfil the overarching aim of delivering a universal recombinant protein vaccine for A. veronii affecting European seabass. Results emerging from the project will help find the chief proteins or genes that affect the virulence of the bacteria and their functions and relationships, as well as ultimately identify new virulence factors, especially for those pathogens that are antigenically diverse. This project is in line with the EU strategy for the sustainable development of aquaculture.

水产养殖系统内的疾病已导致重大经济和生产损失，是扩大可持续水产养殖的主要障碍之一。疫苗接种被广泛认为是防治水产养殖中病原性疾病的有效策略，也是抗生素的有价值的替代品。一种由韦罗尼气单胞菌引起的新出现的细菌感染已经成为爱琴海黑鱼养殖场重大死亡的原因。在体内筛选新的毒力因子可能是确定韦罗尼气单胞菌疫苗开发的潜在候选疫苗的一种方法。一种新的免疫筛选技术，体内诱导抗原技术(IVIAT)，一种依赖于吸附在病原体体外培养上的抗体的方法，用于在感染期间识别细菌上仅在体内表达的免疫原蛋白。这项研究将利用尖端技术来识别宿主体内特定表达或上调的基因。在体内诱导(IVI)蛋白的三个分支共同影响欧洲黑鲈将被选择，以设计一种通用的重组蛋白疫苗感染影响地中海的欧洲黑鲨。该项目的多学科性质很强，涉及微生物学、鱼类免疫学、分子生物学、鱼类疫苗学、鱼类健康和水产养殖。这一跨学科方法对于实现为影响欧洲鲈鱼的韦罗尼弧菌提供通用重组蛋白疫苗的总体目标是必要的。该项目的结果将有助于找到影响细菌毒力及其功能和关系的主要蛋白质或基因，并最终确定新的毒力因素，特别是对那些抗原性多样化的病原体。该项目符合欧盟水产养殖业可持续发展战略。