Development of an innovative recombinant protein vaccine against Aeromonas veronii in European seabass using "omics" technologies (AeroVeroVacc)

使用“组学”技术开发针对欧洲鲈鱼维氏气单胞菌的创新重组蛋白疫苗 (AeroVeroVacc)

• 批准号: EP/Y026454/1
• 负责人: Kimberly Thompson
• 金额: $ 29.8万
• 依托单位: Moredun Research Institute
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY026454%2F1
• 关键词: Development; innovative; recombinant; protein; vaccine

Disease within aquaculture systems has led to significant economic and production losses and is one of the main barriers to expanding sustainable aquaculture. Vaccination is widely accepted as an effective strategy to combat pathogenic diseases in aquaculture and a valuable alternative to antibiotics. An emerging bacterial infection caused by Aeromonas veronii has been the cause of significant mortalities in seabass farms in the Aegean Sea. Screening for novel virulence factors in vivo may be one approach for identifying potential vaccine candidates for vaccine development against Aeromonas veronii. A novel immuno-screeningtechnique, in vivo induced antigen technology (IVIAT), a method that relies on antibodies adsorbed against in vitro cultures of the pathogen, is used to identify immunogenic proteins on bacteria exclusively expressed in vivo during infection. This study will utilise cutting-edge technologies to identify genes specifically expressed or upregulated in vivo within the host. In vivo induced (IVI) proteins common to the three clades of A. veronii affecting European seabass will be selected to design a universal recombinant protein vaccine for A. veronii infections affecting European seabass in the Mediterranean. The project's multidisciplinary nature is strong, involving a combination of microbiology, fish immunology, molecular biology, fish vaccinology, fish health, and aquaculture.This interdisciplinarity approach is necessary to fulfil the overarching aim of delivering a universal recombinant protein vaccine for A. veronii affecting European seabass. Results emerging from the project will help find the chief proteins or genes that affect the virulence of the bacteria and their functions and relationships, as well as ultimately identify new virulence factors, especially for those pathogens that are antigenically diverse. This project is in line with the EU strategy for the sustainable development of aquaculture.

水产养殖系统内的疾病导致了重大的经济和生产损失，是扩大可持续水产养殖的主要障碍之一。疫苗接种被广泛认为是对抗水产养殖病原性疾病的有效策略，也是抗生素的有价值的替代品。由Veronii气单胞菌引起的一种新出现的细菌感染是爱琴海鲈鱼养殖场重大死亡的原因。筛选新的毒力因子在体内可能是一种方法，以确定潜在的疫苗候选疫苗开发针对气单胞菌veronii。体内诱导抗原技术（IVIAT）是一种新的免疫筛选技术，它依赖于体外培养的病原体所吸附的抗体，用于鉴定细菌感染过程中体内表达的免疫原性蛋白。这项研究将利用尖端技术来鉴定宿主体内特异性表达或上调的基因。在体内诱导（IVI）蛋白共同的三个分支A。将选择影响欧洲鲈鱼的A. veronii设计通用的重组蛋白疫苗。影响地中海欧洲鲈鱼的veronii感染。该项目的多学科性质很强，涉及微生物学，鱼类免疫学，分子生物学，鱼类疫苗学，鱼类健康和水产养殖的组合。影响欧洲鲈鱼。该项目的结果将有助于发现影响细菌毒力的主要蛋白质或基因及其功能和关系，并最终确定新的毒力因子，特别是对于抗原多样性的病原体。该项目符合欧盟水产养殖可持续发展战略。