A Shared Neuroscience Platform for National Dissemination and Training in Brain Organogenesis, Behavioral and Brain Disease Models, Viral Vectors, and Imaging Technologies

一个共享神经科学平台，用于脑器官发生、行为和脑疾病模型、病毒载体和成像技术的全国传播和培训

• 批准号: 10647786
• 负责人: William T Newsome
• 金额: $ 136.34万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-16 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647786
• 关键词: 3-Dimensional; Acceleration; Aging; Animals; Area; Array tomography; Autopsy; Basic Science; Behavior; Behavioral; Behavioral Model; Biological Assay; Biotechnology; Brain; Brain Diseases; Brain region; Cell Communication; Cell Line; Cell model; Cells; Central Nervous System Diseases; Charge; Clinical; Clinical Research; Collaborations; Communities; Complementary DNA; Complex; Country; Custom; Development; Disease Progression; Disease model; Educational process of instructing; Educational workshop; Emerging Technologies; Evaluation; Funding; Generations; Genes; Genetic; Genetic Models; Genomics; Goals; Grant; Human; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Industry Standard; Institution; International; Investigation; Investments; Laboratories; Learning; Longitudinal Studies; Maintenance; Memory; Methods; Microscopy; Mission; Missouri; Modality; Modeling; Molecular; Mus; Nervous System; Neurodegenerative Disorders; Neurosciences; Neurosciences Research; Opiate Addiction; Organogenesis; Organoids; Participant; Pathology; Peer Review; Pennsylvania; Persons; Phenotype; Pilot Projects; Plasmids; Preparation; Production; Proteomics; Publications; Reagent; Recombinant Proteins; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Resource Sharing; Resources; Rodent; Running; Scholarship; Scientist; Services; Signal Transduction; Site; Slice; Standardization; Stem Cell Research; Stroke; System; Tauopathies; Techniques; Technology; Testing; Texas; Three-Dimensional Imaging; Time; Tissues; Touch sensation; Training; Transgenic Organisms; Translational Research; Transplantation; United States National Institutes of Health; Universities; Validation; Viral; Viral Vector; Virus; Vision; Visualization; Work; base; behavior test; behavioral phenotyping; behavioral study; brain tissue; clinically relevant; cloud based; computerized data processing; drug candidate; efficacy study; experimental study; functional outcomes; genetic manipulation; hands on instruction; human pluripotent stem cell; imaging system; improved; in vitro Model; in vivo; innovation; innovative technologies; knock-down; member; molecular dynamics; neuroimaging; neuronal circuitry; new technology; novel; novel strategies; novel therapeutics; optogenetics; outreach; programs; self organization; sharing platform; social; three dimensional cell culture; three dimensional structure; tool; underrepresented minority student; vector; viral gene delivery; web site

Advances in neuroscience depend on robust in vivo and in vitro models with innovative technologies to carry out functional and mechanistic studies accompanied by advanced imaging techniques. The Human Brain Organogenesis Program (HBOP), Behavioral and Functional Neuroscience Laboratory (BFNL), Gene Vector and Virus Core (GVVC), and Neuroscience Microscopy Services (NMS) make up a platform, the Stanford Neuroscience Research Center (SNRC), for centralization and dissemination of innovative neuroscience models, reagents and methods. The vision of SNRC is to provide an integrated platform in which users can expand their research to areas outside their expertise or engage multiple modalities in their research, such as applying viral vector approaches and neuroimaging approaches to behavioral models of disease or human organoid cultures. SNRC outreach will approach Neuroscience departments nationally with a call for applications for 6 fully-funded merit-based pilot studies designed to engage diverse SNRC resources. We will also offer comprehensive workshops on techniques and teach participants how to apply and integrate novel approaches into their current research programs. SNRC is strategically equipped with resources to provide critical support to a range of national research projects and has supported over 500 labs nationwide with over 200 peer-reviewed publications in the last decade. SNRC has a growing national user base from institutions including Yale, Harvard, University of Missouri, Cornell, Princeton, Columbia, University of Pennsylvania, University of Texas, MIT, and many more. Investigators anywhere in the world can request a viral vector, phenotype rodent lines, or have an in vivo stroke study or an efficacy study in a model of neurodegenerative disease run remotely. Anyone can attend a workshop for training in behavioral models, 3D imaging of whole brains, or methods for 3D human cellular models (organoids and assembloids). SNRC supports many small and large biotech companies in proof of concept efficacy studies of clinical drug candidates. This contribution has supported the advancement of these projects to human clinical studies. Through SNRC, academic users will have access to these same industry- standard efficacy studies. All SNRC activities will be supervised by a steering committee consisting of external, internal, and NIH members. Under this U24 grant, we will disseminate essential and cutting-edge resources. We will expand our external user network to share emerging technologies with the Pilot Study program and Annual Workshops. BFNL will expand its automated testing and data processing capabilities, including the Digilab cloud- based automated behavioral phenotyping system. GVVC will set up large-scale viral production as well as higher-level purification technology. NMS will include training classes in STARmap genomic imaging and array tomography proteomic imaging. Under this program, SNRC will engage with the national neuroscience community via newly improved resource sharing websites, hands-on instruction, and annual in-person or online workshops to facilitate networks for collaboration and acceleration of discoveries in brain function and pathology.

神经科学的进步依赖于强大的体内和体外模型以及创新技术来进行 功能和机制研究伴随先进的成像技术。人脑 器官发生计划 (HBOP)、行为和功能神经科学实验室 (BFNL)、基因载体 病毒核心（GVVC）和神经科学显微镜服务（NMS）组成了一个平台，斯坦福大学 神经科学研究中心 (SNRC)，用于集中和传播创新神经科学 模型、试剂和方法。 SNRC 的愿景是提供一个集成平台，用户可以在其中 将研究扩展到其专业知识之外的领域或在研究中采用多种模式，例如 将病毒载体方法和神经影像方法应用于疾病或人类的行为模型 类器官培养物。 SNRC 外展活动将联系全国神经科学部门并征集申请 6 项全额资助的择优试点研究，旨在利用不同的 SNRC 资源。我们还将提供 综合技术研讨会，教授参与者如何应用和整合新方法 纳入他们当前的研究计划。 SNRC 在战略上配备了资源，可以为 一系列国家研究项目，并支持全国 500 多个实验室，并经过 200 多个同行评审 近十年来的出版物。 SNRC 拥有不断增长的全国用户群，来自耶鲁大学、哈佛大学、 密苏里大学、康奈尔大学、普林斯顿大学、哥伦比亚大学、宾夕法尼亚大学、德克萨斯大学、麻省理工学院和 还有更多。世界任何地方的研究人员都可以请求病毒载体、表型啮齿动物品系，或者拥有 体内中风研究或远程运行的神经退行性疾病模型的功效研究。任何人都可以 参加行为模型、全脑 3D 成像或 3D 人体方法培训研讨会 细胞模型（类器官和组合体）。 SNRC 支持许多小型和大型生物技术公司的事实证明 临床候选药物的概念功效研究。这一贡献支持了这些领域的进步 人类临床研究项目。通过 SNRC，学术用户将能够接触到这些相同的行业- 标准功效研究。 SNRC 的所有活动将由一个指导委员会监督，该委员会由外部、 内部和 NIH 成员。根据这笔 U24 赠款，我们将传播重要和前沿的资源。我们 将扩大我们的外部用户网络，通过试点研究计划和年度研究分享新兴技术 研讨会。 BFNL 将扩展其自动化测试和数据处理能力，包括 Digilab 云- 基于自动化行为表型系统。 GVVC将建立大规模病毒生产以及 更高级别的净化技术。 NMS 将包括 STARmap 基因组成像和阵列培训课程 断层扫描蛋白质组成像。根据该计划，SNRC 将与国家神经科学领域合作 通过新改进的资源共享网站、实践指导以及年度面对面或在线社区 举办研讨会，以促进合作网络并加速脑功能和病理学的发现。