IMAGINE - Ion beaM Analysis to decipher the bioloGical response Induced by Nanoplastics at Environmentally realistic concentration

想象 - 离子束分析可破译纳米塑料在环境实际浓度下引起的生物反应

• 批准号: EP/Z000629/1
• 负责人: Maya Al-Sid-Cheikh
• 金额: $ 273.65万
• 依托单位: University of Surrey
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FZ000629%2F1
• 关键词: IMAGINE; Ion; beaM; Analysis; decipher

Studies investigating the effects of nanoplastics (NPs) on aquatic organisms used concentrations between 2 to 7 order-of-magnitudes higher than those predicted in the open ocean. These studies divided the community between those sounding the alarm due to the observed ecotoxicological effects, and those predicting that NP concentrations in the environment are far below any threshold-effect.Most experiments were inadequately designed, and thus the results unsatisfying. Fit-to-purpose experimental designs have been hindered by a lack of appropriate NP models, analytical methods, and monitoring strategies for predicted NP concentrations. Using [14C]NPs and conventional nuclear techniques, I have recently modelled that scallops, chronically exposed (> 1 y) to environmentally realistic NP concentrations (15 .........g/L) might accumulate and reach NPs body burden where effects are observed by those sounding the alarm. Astonishingly, this suggests that NPs might already be beyond threshold-effects in organisms and harmingthe marine biota.Here, I propose an innovative approach that will overcome the analytical limitations to correlate potential local biological responses with mapping and quantification of NPs under realistic environmental settings. By developing [14C]NPs of the most produced plastics, and combining then with the analytical power of the accelerator mass spectrometry, ion beam analysis and mass spectrometry imaging, IMAGINE will answer whether NPs in the oceans are already beyond "threshold-effect" concentrations? This novel analytical approach will provide a unique insight into the potential effects of NPs following chronic exposures and will:1) provide key intrinsically radiolabelled NP models;2) develop an analytical suite to generate spatially-resolved toxicokinetic and metabolomic data;3) perform chronic NPs exposures at predicted NPs concentrations;4) answer whether key NPs accumulate and induce local biological responses at predicted concentration

调查纳米塑料（NP）对水生生物影响的研究使用的浓度比公海中预测的浓度高出2至7个数量级。这些研究将社区划分为由于观察到的生态毒理学效应而发出警报的社区和预测环境中NP浓度远低于任何阈值效应的社区。由于缺乏合适的NP模型、分析方法和预测NP浓度的监测策略，因此无法进行符合目的的实验设计。使用[14 C] NP和常规核技术，我最近模拟了扇贝，长期暴露（> 1年）于环境现实的NP浓度（15...... g/L）可能会积累并达到NPs的体内负荷，其中那些发出警报的人观察到了影响。令人惊讶的是，这表明，纳米粒子可能已经超出了阈值的影响，在生物体和harmingthe海洋biota.Here，我提出了一个创新的方法，将克服分析的局限性，潜在的本地生物反应与映射和量化的纳米粒子在现实的环境设置。通过开发生产最多的塑料的[14 C] NPs，并结合加速器质谱分析，离子束分析和质谱成像的分析能力，IMAGINE将回答海洋中的NPs是否已经超过“阈值效应”浓度？这种新的分析方法将为长期暴露后NP的潜在影响提供独特的见解，并将：1）提供关键的固有放射性标记NP模型; 2）开发分析套件以生成空间分辨的毒代动力学和代谢组学数据; 3）在预测的NP浓度下进行长期NP暴露; 4）回答关键NP是否在预测浓度下蓄积并诱导局部生物学反应