GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY

我们用简并引物对酵母菌和水母的DNA进行了聚合酶链式反应 确定与甲状腺/类固醇核家族基因同源的基因 感受器。我们已经克隆并部分测序了五个不同的基因 似乎与甲状腺/类固醇家族同源的酵母。一个 另外20个已经被鉴定出来，它们在引物中几乎相同 地区，但在其他地方有些不同。其中一些将被克隆 并进行了测序。对水母DNA的初步实验表明， 凝胶上的条带，其中一条大小正好适合锌指蛋白。

We have used degenerate primers in PCR of DNA from yeast and medusa to identify genes homologous to those in the thyroid/steroid family of nuclear receptors. We have cloned and partially sequenced five different genes from yeast that appear to be homologous to the thyroid/steroid family. An additional 20 have been identified which are almost identical in the primer region but are somewhat divergent elsewhere. Some of these will be cloned and sequenced. Preliminary experiments with medusa DNA show after PCR, bands on a gel, one of which is the right size for a zinc finger protein.