GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
批准号:
5202026
负责人:
J E RALL
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
我们小组一直在调查甲状腺/类固醇激素的成员
英文摘要
Our group has been investigating members of thyroid/steroid hormone
receptor family in lower organisms in an attempt to analyze the structure
and functions of the most primitive receptors, better to understand the
function of the myriad of receptors present in the vertebrata. We cloned
cDNAs for three such proteins in the nematode Caenorhabditis elegans. One
of them (CHR3) is highly homologous to the DHR3 in Drosophila and MHR3
in Manduca sexta. This receptor in Drosophila is induced by ecdysone.
Another receptor we cloned (CNR14) is homologous to E78A receptor, also
ecdysone inducible in Drosophila. The third receptor, CNR8 is similar
to an early response gene in vertebrates, NAK1. The receptors are stage
specific as shown by Northern analysis. More recently, we have cloned the
genes for CHR3 and CNR14 and we are studying their promoters. We found
that two genes, CHR3 and CNR14 are trans-spliced and so are organized in
operons. While CHR3 is trans-spliced with a leader sequence SL2, and
CNR14 is trans-spliced with SL1, CNR8 seems to be transcribed as single
transcript. Cistronic transcription gives us the opportunity to study
the genes which are transcribed from a common promoter and which could
function together. We mapped CHR3 to the center region of chromosome 1
in close relation to hlh-2 gene, a homeobox protein analogous to E12
protein, the partner of MyoD in vertebrates. We confirmed the
propinquity of the hlh-2 and CHR3 by PCR on a cosmid. Interestingly, some
known mutants with deletion in this region are lethal. We are currently
studying expression of CHR3 and CNR14 using microinjetions to the ovary
of hermaphrodites with probable promoter regions coupled to DNA sequence
coding for beta-galactosidase. We also hope to use heterozygotic strains
with deletions in a region where CHR3 maps for a study of knock-out and
rescue.
We continued on the steroid hormone receptor in cubomedusan jellyfish
Tripedalia cystophora. We cloned at least two cDNA isoforms and most of
the gene. As far as we can tell, this is the only such gene in his
organism and could represent the most primitive member of
thyroid/retinoic acid subfamily.
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GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
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批准号:3776931
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J E RALL
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依托单位:
GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
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批准号:6162006
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J E RALL
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依托单位:
GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
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批准号:3754849
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:J E RALL
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依托单位:
GENES ANCESTRAL TO THE THYROID/STEROID RECEPTOR FAMILY
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批准号:2573456
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:J E RALL
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依托单位:
海外基金