TRANSITION-METAL MEDIATED FREE RADICAL FORMATION IN VITRO AND IN VIVO

越来越多的证据表明，可能大多数急性和慢性金属 中毒涉及组织损伤中的自由基反应。 以来 在身体组织中发生的自由基的浓度通常接近 为了克服ESR谱的灵敏度极限，使用了自旋捕获技术。 对动物模型的操作也提供了证据， 毒性确实是自由基介导的。 通过成功应用这一 方法，ESR证据已获得自由基的形成 产生的铁可能发生在体内，不仅急性后， 慢性铁摄入 铬（VI）化合物对职业健康构成严重危害， 其潜在的呼吸道致癌性及其 对其他器官的毒性作用。 通过应用ESR和自旋捕获 技术，自由基加合物的存在也已显示在胆汁中 大鼠急性Cr（VI）中毒后。 进行额外测量 以提供更多关于确切机制的数据， 由Cr（VI）中毒形成的检测到的自由基。 我们没有 能够显示胆汁或不同组织（脑， 肝脏、肾脏） 酸 我们还发现铅和铁或铝缺乏毒性协同效应 和铁元素的含量

Increasing evidence indicates that probably most acute and chronic metal poisoning involve free radical reactions in tissue injury. Since the concentration of radicals occurring in body tissues is generally near the sensitivity limit of ESR spectroscopy, the spin trapping technique is used. The manipulation of an animal model also provides evidence that the toxicity is indeed free radical mediated. By successfully applying this approach, ESR evidence has been obtained for free radical formation generated by iron may occur in vivo not only after acute but also after chronic iron intake. Chromium (VI) compounds pose a serious occupational health hazard in terms of both their potential respiratory tract carcinogenicity and their toxic effect on other organs. By applying ESR and a spin trapping technique, the existence of a radical adduct has also been shown in bile of rats after acute Cr(VI) poisoning. Additional measurements are carried out to provide more data about the exact mechanism by which the detected free radicals from Cr(VI) intoxication are formed. We were not able to show free radical formation in bile or in different tissues (brain, liver, kidneys) after poisoning of rats with lead or with aminolevulinic acid. We also found lack of toxic synergistic effects of Pb and Fe or Al and Fe after these acute metal-metal poisonings.