SUBTLE SEQUENCE PATTERNS IN DNA-BINDING COMPLEXES

DNA 结合复合物中的微妙序列模式

• 批准号: 3781283
• 负责人: J WOOTTON
• 金额: --
• 依托单位: NATIONAL LIBRARY OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3781283
• 关键词: DNA binding protein; automated data processing; computer assisted sequence analysis; computer system design /evaluation; conformation; method development; protein sequence; protein structure; statistics /biometry; transcription factor

The number of classes of proteins that recognize specific sequences and structures of DNA is continuing to grow rapidly. Several are large complexes of several types of subunit. In this project, computational strategies are being developed to recognize some of the functional amino acid sequence patterns involved, many of which are subtle and variable. Improved methods are being applied to the analysis of new sequences determined in other NIH laboratories. For patterns of well-established DNA binding regions such as helix-turn-helix and basic regions, new methods for evaluation of the diagnostic power of pattern discriminators are being developed and applied to more novel patterns. Low- complexity sequences are frequent in DNA-binding proteins and require analysis and filtering before database searches. The new method of automated local multiple alignment by iterative sampling is also included in these strategies. Specific sequences analyzed include (1) 127 kDa component of a UV-damaged-DNA binding complex, which is defective in some Xeroderma Pigmentosum Group E patients and shows sequence similarities to proteins of unknown function from a wide range of eukaryotes, and (2) six subunits of the transcription factor TFIID complex that is central in transcriptional regulation by facilitating promoter responses to various activators.

识别特定序列的蛋白质种类的数量， DNA的结构正在快速增长。 有几个很大 几种类型的亚基的复合物。在这个项目中，计算 正在制定战略，以认识到一些功能 涉及的氨基酸序列模式，其中许多是微妙的， 变量 改进后的方法正被用于分析新的 其他NIH实验室确定的序列。 对于已确立的DNA结合区域的模式，如 螺旋-转角-螺旋和基本区域， 模式鉴别器的诊断能力正在开发中， 应用到更新颖的模式上。 低复杂度的序列 在DNA结合蛋白中， 数据库搜索 自动局部多次波新方法 这些策略中还包括通过迭代采样进行的对齐。 分析的特异性序列包括（1）127 kDa组分， 紫外线损伤的DNA结合复合物，这是有缺陷的一些干皮病 色素性E组患者，并显示与蛋白质的序列相似性 的未知功能，从广泛的真核生物，和（2）六个 转录因子TFIID复合物的亚基，其在 通过促进启动子对多种转录因子的应答来进行转录调控 活化剂。