SUBTLE SEQUENCE PATTERNS IN DNA-BINDING COMPLEXES

识别特定序列的蛋白质类别的数量和 DNA的结构继续快速增长。有几个是大的 几种亚基的复合体。在这个项目中，计算性 正在制定策略，以识别一些功能 涉及氨基酸序列模式，其中许多是微妙的和 变量。改进的方法正被应用于分析新的 在其他NIH实验室中确定的序列。 对于已建立的DNA结合区的模式，例如 螺旋-转角-螺旋和基本区域，新的评估方法 模式鉴别器的诊断能力正在开发中，并且 应用于更新颖的图案。低复杂度序列是常见的 在DNA结合蛋白中，需要分析和过滤之前 数据库搜索。自动局部多次波的新方法 通过迭代抽样进行比对也包括在这些策略中。 分析的特定序列包括(1)127 kDa组分 紫外线损伤的DNA结合复合体，在一些干皮病中存在缺陷 E组患者的色素沉着症，并显示出与蛋白质的序列相似性 来自广泛的真核生物的未知功能，以及(2)六 转录因子TFIID复合体的亚基，在 通过促进启动子对不同基因的反应进行转录调控 激活剂。

The number of classes of proteins that recognize specific sequences and structures of DNA is continuing to grow rapidly. Several are large complexes of several types of subunit. In this project, computational strategies are being developed to recognize some of the functional amino acid sequence patterns involved, many of which are subtle and variable. Improved methods are being applied to the analysis of new sequences determined in other NIH laboratories. For patterns of well-established DNA binding regions such as helix-turn-helix and basic regions, new methods for evaluation of the diagnostic power of pattern discriminators are being developed and applied to more novel patterns. Low- complexity sequences are frequent in DNA-binding proteins and require analysis and filtering before database searches. The new method of automated local multiple alignment by iterative sampling is also included in these strategies. Specific sequences analyzed include (1) 127 kDa component of a UV-damaged-DNA binding complex, which is defective in some Xeroderma Pigmentosum Group E patients and shows sequence similarities to proteins of unknown function from a wide range of eukaryotes, and (2) six subunits of the transcription factor TFIID complex that is central in transcriptional regulation by facilitating promoter responses to various activators.