MOLECULAR NOVELTY AND CONSERVATION IN BACTERIAL PROTEIN SEQUENCES

细菌蛋白质序列的分子新颖性和保守性

• 批准号: 3845114
• 负责人: J WOOTTON
• 金额: --
• 依托单位: NATIONAL LIBRARY OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3845114
• 关键词: Archaea; Myxococcus; Streptomyces; Yersinia pestis; bacterial proteins; biochemical evolution; genome; intermolecular interaction; microorganism classification; nucleic acid sequence; photosynthetic bacteria; protein kinase; protein kinase C; protein sequence; protein structure function; serine proteinases

Protein sequences deduced from gene sequences of diverse bacteria and archaebacteria are yielding a wealth of new knowledge on protein functions, interactions and evolution. A number of unexpected novelties were studied by concerted methods including directed mutagenesis, spectroscopic/enzymological analyses and computer analyses of sequence databases. Findings include:- A. Bacterial homologs of major "eukaryotic" protein families. The antiquity of Serine/Threonine protein kinases was confirmed by discovery of protein kinase C homologs in cyanobacteria and archaebacteria, in addition to those already known to be involved in cellular differentiation in Myxococcus. TPR repeats characteristic of many cell cycle proteins in eukaryotes were found in different cyanobacteria and Yersinia enterocolitica. B. Distinct bacterial and eukaryotic molybdoproteins. At the sequence level, two completely distinct, strongly conserved families of pterin-molybdenum cofactor enzymes perform the same hydroxylation and redox reactions in (a) archaebacteria plus eubacteria and (b) eukaryotes. Sequence features were correlated with protein spectroscopic properties and domain organization. C. Sequence families in complex bacteria. Available sequences from multicellular and differentiating bacteria, Streptomyces, Myxococcus and various cyanobacteria and archaebacteria, were classified by global database sequence similarity searches. More than 50 percent of the classifiable protein sequences did not have counterparts in E. coli and other well-studied enteric bacteria, and many of these had eukaryotic homologs. Significance of project: Genome sequences from metabolically and morphogenetically diverse bacteria provide a rich and cost-effective source of new discoveries on the molecular functions and evolution of proteins.

从多种细菌的基因序列推导出的蛋白质序列 古细菌正在产生大量有关蛋白质的新知识 功能、相互作用和进化。 许多意想不到的新奇事物 通过一致的方法进行研究，包括定向诱变， 光谱/酶学分析和序列计算机分析 数据库。调查结果包括：- A. 主要“真核”蛋白质家族的细菌同源物。 这 发现证实了丝氨酸/苏氨酸蛋白激酶的古老性 蓝藻和古细菌中蛋白激酶 C 同源物的研究 除了那些已知参与细胞分化的物质之外 在粘球菌属中。 TPR 重复了许多细胞周期蛋白的特征 在不同的蓝细菌和耶尔森氏菌中发现了真核生物 小肠结肠炎。 B. 不同的细菌和真核钼蛋白。 在顺序上 水平上，两个完全不同的、高度保守的家族 蝶呤-钼辅因子酶具有相同的羟基化作用 (a) 古细菌加真细菌和 (b) 真核生物中的氧化还原反应。 序列特征与蛋白质光谱特性相关 和域组织。 C. 复杂细菌中的序列家族。 可用序列来自 多细胞和分化细菌、链霉菌、粘球菌和 各种蓝细菌和古细菌，按全球分类 数据库序列相似性搜索。 超过50%的 可分类的蛋白质序列在大肠杆菌中没有对应物，并且 其他经过充分研究的肠道细菌，其中许多具有真核细菌 同系物。 项目意义：代谢和基因组序列 形态发生多样化的细菌提供了丰富且具有成本效益的 分子功能和进化新发现的来源 蛋白质。