ADVERSE ALLERGIC REACTIONS TO VACCINES

酶联免疫吸附测定（ELISA）掺入小鼠 作为捕获抗体和二抗的抗人IgE单克隆抗体已被 检测人血清样品中总IgE的测定。 试剂 测试提供了准确的，可重复的读数使用内部 已知IgE含量的标准人血清池。 从JEV获得的血清 参与美国海军免疫计划和JEV的反应堆 在丹麦免疫的反应器中检测总IgE含量。 鼠疫疫苗免疫前后血清 还检查了个体的总IgE。的试验开发 检测疫苗和疫苗组分的特异性IgE， 启动。 鼠疫疫苗特异性IgE的检测已表明 在有限的化验中。

An enzyme linked immunosorbant assay (ELISA) incorporating mouse anti-human IgE monoclonals as capture and secondary antibodies has been tested for measurement of total IgE in human serum samples. Reagents tested have provided accurate, reproducible readings using an in-house standard human serum pool with known IgE content. Sera obtained from JEV reactors participating in the U.S. Navy immunization program and from JEV reactors immunized in Denmark have been examined for total IgE content. Pre and post-immunization sera obtained from plague vaccine immunized individuals have also been examined for total IgE. Assay development for detection of specific IgE to vaccines and vaccine components has been initiated. Detection of plague vaccine specific IgE has been indicated in limited assays.