SYNTHESIS OF OLIGONUCLEOTIDES

寡核苷酸由细胞生理学实验室合成 与DBP各实验室的调查人员合作 并与FDA以外发起的研究项目相结合。这些 寡核苷酸是根据个人的特定需要量身定做的 研究人员，并在广泛的研究项目中使用。 DBP的研究领域包括大肠杆菌胶囊的克隆和测序 分枝杆菌抗原基因的DNA测序及其作为引物的应用 用于开发一种结合聚合酶链的诊断方法 确定感染与百日咳的反应。目前的研究也是 正在进行的是确定药物代谢酶的水平在 对疫苗和其他制剂的反应，并产生选择性突变 以及细菌毒素基因的扩增，以研究其作用 毒素功能上的突变。 我们实验室的研究包括使用寡核苷酸作为工具 表位文库的创建。在合作研究中与 在土耳其和日本的研究人员，我们的寡核苷酸正被应用于 分枝杆菌的诊断和毒鼠强的分离探讨 PS-12细胞的受体基因。

Oligonucleotides are synthesized by the Laboratory of Cellular Physiology in collaboration with investigators from the various laboratories in DBP and in conjunction with research projects initiated outside of FDA. These oligonucleotides are tailored to fit the specific needs of the individual investigators, and are being used in a wide variety of research programs. Areas of research in DBP include cloning and sequencing of E. coli capsule genes, DNA sequencing of Mycobacterial antigen genes, and use as primers for development of a diagnostic method incorporating Polymerase Chain Reaction to identify infection with Pertussis. Current research is also being conducted to determine the levels of drug metabolizing enzymes in response to vaccines and other agents, and to produce selective mutation and amplification of bacterial toxin genes in order to study the effect of the mutation on toxin function. Studies in our laboratory include the use of oligonucleotides as tools for the creation of an epitope library. In collaborative studies with researchers in Turkey and Japan, our oligonucleotides are being applied as probes for the diagnostics of Mycobacterium and the isolation of muscarinic receptor genes from PS-12 cells.