SYNTHESIS OF OLIGONUCLEOTIDES

本实验室合作合成寡核苷酸 与细菌产品部门内的几个实验室合作， 被证明对以下研究项目非常宝贵：PCR方法， 克隆并表达肺炎支原体潜在表面抗原， 并修改现有的克隆载体。开发一种方法， 用于PCR生成产物的直接克隆，以及作为DNA的模板 支原体测序。完成核苷酸序列分析 编码几种免疫反应性抗原的分枝杆菌基因。 从两种基因组中分离肺炎球菌溶血素基因 DNA，以产生肺炎球菌素基因的截短点突变，和 用于单一多糖的缀合。 继续研究 细菌毒素的选择性突变和扩增的产生 用于检测突变对毒素功能的影响的基因。 引物也被用于PCT协议的开发，以快速地 检测非结核分枝杆菌感染。 我们实验室正在进行利用克隆 重叠链，以开发体外转录探针， 苹果过敏原筛选，确定并分离过敏原 成分研究还继续包括使用 因此，本发明提供了寡核苷酸作为用于产生表位文库的工具。

Synthesis of oligonucleotides by our laboratory in collaborative efforts with several laboratories within the Division of Bacterial Products has proven invaluable to the following research programs: A PCR approach to clone and express a potential surface antigen from Mycoplasma pneumonia, and to modify existing cloning vectors. Development of a method to allow for direct cloning of PCR generated products, and as templates for DNA sequencing in Mycoplasma. Completion of the nucleotide sequence analysis of mycobacterial genes encoding several immunoreactive antigens. Isolation of the pneumococcal pneumolysin gene from two types of genomic DNA, to produce trunicated point mutations of the pneumolynsin gene, and for conjugation of a single polysaccharide. Continued research in the production of selective mutation and amplification of bacterial toxin genes for use in examining the effect of mutation on toxin function. Primers were also utilized in the development of PCT protocols to rapidly detect non tuberculosos mycobacterial infections. Research in our laboratory is being conducted in the use of cloned overlapping strnads, to develop an in vitro transcription probe for allergenic screening of apple to determine and isolate the allergenic component. Studies are also continuing to include the use of oligonucleotides as tools for the creation of an epitope library.